Literature summary for 7.4.2.6 extracted from

Yoon, H.J.; Kim, H.J.; Mikami, B.; Yu, Y.G.; Lee, H.H.
Crystal structure of a putative oligopeptide-binding periplasmic protein from a hyperthermophile (2016), Extremophiles, 20, 723-731 .

Search for more literature for 7.4.2.6

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 100 mM HEPES pH 7.5, 1.0 M trisodium citrate	Thermotoga maritima

Protein Variants

Protein Variants	Comment	Organism
V57P/W551P	the mutant essentially shows similar peptide-binding affinity compared to the wild type enzyme	Thermotoga maritima

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
periplasm	-	Thermotoga maritima	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
73	-	gel filtration	Thermotoga maritima

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	Q9WXR2	-	-
Thermotoga maritima ATCC 43589	Q9WXR2	-	-

Purification (Commentary)

Purification (Comment)	Organism
Sepharose column chromatography, and Superdex 200 gel filtration	Thermotoga maritima

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + GGGQATGPPNTSYMPGMPGD-[GGGQATGPPNTSYMPGMPGD-binding protein][side 1]	-	Thermotoga maritima	ADP + phosphate + GGGQATGPPNTSYMPGMPGD[side 2] + [GGGQATGPPNTSYMPGMPGD-binding protein][side 1]	-	?
ATP + H2O + GGGQATGPPNTSYMPGMPGD-[GGGQATGPPNTSYMPGMPGD-binding protein][side 1]	-	Thermotoga maritima ATCC 43589	ADP + phosphate + GGGQATGPPNTSYMPGMPGD[side 2] + [GGGQATGPPNTSYMPGMPGD-binding protein][side 1]	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 71000, SDS-PAGE	Thermotoga maritima
monomer	1 * 73000, calculated from amino acid sequence	Thermotoga maritima

Synonyms

Synonyms	Comment	Organism
oligopeptide ABC transporter	-	Thermotoga maritima
oligopeptide-binding protein	-	Thermotoga maritima
OppA	-	Thermotoga maritima

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Release 2023.1 (January 2023)