Literature summary for 7.4.2.6 extracted from

Berntsson, R.P.; Doeven, M.K.; Fusetti, F.; Duurkens, R.H.; Sengupta, D.; Marrink, S.J.; Thunnissen, A.M.; Poolman, B.; Slotboom, D.J.
The structural basis for peptide selection by the transport receptor OppA (2009), EMBO J., 28, 1332-1340.

Crystallization (Commentary)

Crystallization (Comment)	Organism
OppA in the open-and closed-liganded conformation, complexed with bradykinin, the nonapeptide RPPGFSPFR, hanging drop vapour diffusion method, 0.001 ml of protein solution are mixed with 10 mg/ml ligand-free OppA, 9 mM Na-MES, pH 6.0, 9 mM NaCl and 1 mM peptide, is mixed with 0.001 ml of reservoir solution containing 0.2 M NaCl, 0.1 M Na-HEPES, pH 7.0, and 20% PEG 6000, 12 weeks at room temperature, X-ray diffraction structure determination and analysis at 1.3 and 2.5 A resolution, respectively	Lactococcus lactis

Crystallization (Comment)

Organism

OppA in the open-and closed-liganded conformation, complexed with bradykinin, the nonapeptide RPPGFSPFR, hanging drop vapour diffusion method, 0.001 ml of protein solution are mixed with 10 mg/ml ligand-free OppA, 9 mM Na-MES, pH 6.0, 9 mM NaCl and 1 mM peptide, is mixed with 0.001 ml of reservoir solution containing 0.2 M NaCl, 0.1 M Na-HEPES, pH 7.0, and 20% PEG 6000, 12 weeks at room temperature, X-ray diffraction structure determination and analysis at 1.3 and 2.5 A resolution, respectively

Lactococcus lactis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Lactococcus lactis	the enzyme follows a mechanism for peptide selection based on amino-acid composition rather than sequence, overview	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Lactococcus lactis	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
additional information	OppA is complexed with proline-rich endogenous peptides, MALDI tandem mass spectrometry peptide analysis, overview	Lactococcus lactis

Purification (Commentary)

Purification (Comment)	Organism
OppA without lipid anchor is produced in the cytoplasm of Lactobacillus lactis where endogenous peptides are available for binding. These peptides bind so tightly that they remain associated with the protein throughout the purification, they can be removed from the protein only by guanidium chloride treatment generating ligand-free OppA	Lactococcus lactis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme follows a mechanism for peptide selection based on amino-acid composition rather than sequence, overview	Lactococcus lactis	?	-	?
additional information	OppA binds peptides of an exceptionally wide range of lengths of 4 to 35 residues with no apparent sequence preference. A huge cavity allows binding of very long peptides, and a lack of constraints for the position of the N and C termini of the ligand is compatible with binding of peptides with varying lengths, e.g. bradykinin, RPPGFSPFR	Lactococcus lactis	?	-	?

Subunits

Subunits	Comment	Organism
oligomer	molecular dynamics simulations, with the protein maintaing the overall open structure and shape, and structure modelling	Lactococcus lactis

Synonyms

Synonyms	Comment	Organism
oligopeptide-binding protein A	-	Lactococcus lactis
OppA	-	Lactococcus lactis
transport receptor	-	Lactococcus lactis