Cloned (Comment) | Organism |
---|---|
gene oppA, DNA and amino acid sequence determination and analysis, expression of the wild-type enzyme in Escherichia coli strain BL21(DE3) | Vibrio furnissii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a hly-oppA knockout mutant of Vibrio furnissii, VFYKW1, by the allelic exchange method, the hly-oppA knockout mutant causes less mortality than the wild-type strain when it was inoculated into BALB/c mice, morphology and biofilm production of the hly-oppA knockout mutant, overview | Vibrio furnissii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + olipopeptide/out | Vibrio furnissii | Hly-OppA has multiple functions in solute binding, in in vitro hemolysis, in antibiotic resistance, and as a virulence factor in bacterial pathogenesis, overview | ADP + phosphate + oligopeptide/in | - |
? | |
additional information | Vibrio furnissii | the purified protein exhibits host-specific in vitro hemolytic activity against various mammalian erythrocytes and apparent cytotoxicity in CHO-K1 cells | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio furnissii | Q0PRU9 | gene oppA | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
biofilm formation assay of wild-type and mutant strains | Vibrio furnissii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + olipopeptide/out | - |
Vibrio furnissii | ADP + phosphate + oligopeptide/in | - |
? | |
ATP + H2O + olipopeptide/out | Hly-OppA has multiple functions in solute binding, in in vitro hemolysis, in antibiotic resistance, and as a virulence factor in bacterial pathogenesis, overview | Vibrio furnissii | ADP + phosphate + oligopeptide/in | - |
? | |
additional information | the purified protein exhibits host-specific in vitro hemolytic activity against various mammalian erythrocytes and apparent cytotoxicity in CHO-K1 cells | Vibrio furnissii | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Hly-OppA | - |
Vibrio furnissii |
oligopeptide permease | - |
Vibrio furnissii |