Any feedback?
Literature summary for 7.4.2.5 extracted from
- Expression and purification of Pseudomonas aeruginosa SecA N-terminal domain: stimulation of ATPase activity of the SecAL43P mutant protein (2006), Protein Expr. Purif., 47, 629-633.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the DNA fragment encoding the N-terminal 236 amino acid residues is cloned into the pET20b vector for expression in Escherichia coli BL21.19 cells | Pseudomonas aeruginosa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| PaSecAL43P | mutant | Pseudomonas aeruginosa |
| PaSecAN236 | N-terminal 236 amino acids of PaSecA | Pseudomonas aeruginosa |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasmic membrane | - |
Pseudomonas aeruginosa | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
mutant PaSecAN236, determined by SDS-PAGE | Pseudomonas aeruginosa |
| 102000 | - |
monomer | Pseudomonas aeruginosa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Pseudomonas aeruginosa | - |
ADP + phosphate | - |
ir |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | Q9LCT3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using osmotic shock, a SP-Sepharose 26/10 packed cation-exchange column, and a Sephacryl-200 HR 16/90 packed column | Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.001 | - |
PaSecAN236, liposome | Pseudomonas aeruginosa |
| 0.0036 | - |
PaSecAL43P | Pseudomonas aeruginosa |
| 0.0065 | - |
PaSecAL43P, liposome | Pseudomonas aeruginosa |
| 0.0065 | - |
PaSecAN236, PaSecAL43P | Pseudomonas aeruginosa |
| 0.052 | - |
PaSecAN236, PaSecAL43P, liposome | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Pseudomonas aeruginosa | ADP + phosphate | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | - |
Pseudomonas aeruginosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PaSecA | - |
Pseudomonas aeruginosa |
| SecA | - |
Pseudomonas aeruginosa |
| SecA ATPase | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
ATPase activity assay | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
ATPase activity assay | Pseudomonas aeruginosa |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
