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Literature summary for 7.4.2.5 extracted from

  • Demirel, O.; Waibler, Z.; Kalinke, U.; Gruenebach, F.; Appel, S.; Brossart, P.; Hasilik, A.; Tampe, R.; Abele, R.
    Identification of a lysosomal peptide transport system induced during dendritic cell development (2007), J. Biol. Chem., 282, 37836-37843.
    View publication on PubMed
Search for more literature for 7.4.2.5

Cloned(Commentary)

Cloned (Comment) Organism
human TAPL is cloned into pEGFP-N3 and pIRES2-EGFP resulting in TAPL with a C-terminal EGFP and TAPL co-translated EGFP, respectively, TAPL containing a C-terminal myc-tag is cloned into pcDNA3.1+, and into the retroviral vector pLPCX Homo sapiens

Protein Variants

Protein Variants Comment Organism
K545A/H699A TAPL double mutant Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
orthovanadate
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Homo sapiens 5764
-
membrane
-
 Homo sapiens 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 monomer, determined by SDS-PAGE and immunoblotting Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + peptide/in Homo sapiens
-
 ADP + phosphate + peptide/out
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
BRE-169 cell TAP1-deficient skin fibroblast cell line Homo sapiens
-
Burkitt lymphoma cell
-
 Homo sapiens
-
HEK-293T cell
-
 Homo sapiens
-
HeLa cell
-
 Homo sapiens
-
monocyte TAPL expression is strongly induced during differentiation of monocytes to dendritic cells and to macrophages Homo sapiens
-
STF1-169 cell TAP2-deficient skin fibroblast cell line Homo sapiens
-
THP-1 cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00000022
-
 peptide transport rate, K545A/H699A mutant, in the presence of 3 mM ATP Homo sapiens
0.00000022
-
 peptide transport rate, wild type, in the presence of 3 mM ATP and 1 mM orthovanadate Homo sapiens
0.00000026
-
 peptide transport rate, K545A/H699A mutant, in the presence of 3 mM ATP and 500 microM competitor peptide RRYQKSTEL Homo sapiens
0.00000026
-
 peptide transport rate, wild type, in the presence of 3 mM AMP Homo sapiens
0.0000003
-
 peptide transport rate, K545A/H699A mutant, in the presence of 3 mM ATP and 1 mM orthovanadate Homo sapiens
0.00000035
-
 peptide transport rate, wild type, in the presence of 3 mM ATP and 500 microM competitor peptide RRYQKSTEL Homo sapiens
0.00000039
-
 peptide transport rate, K545A/H699A mutant, in the presence of 3 mM AMP Homo sapiens
0.00000156
-
 peptide transport rate, wild type, in the presence of 3 mM ATP Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + peptide/in
-
 Homo sapiens ADP + phosphate + peptide/out
-
 ?
ATP + H2O + RRYCKSTEL/in substrate in peptide transport assay, fluorescein coupled via a cyteine residue Homo sapiens ADP + phosphate + RRYCKSTEL/out
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 70000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
ABCB9
-
 Homo sapiens
peptide-transporting ATPase
-
 Homo sapiens
TAP-like transporter
-
 Homo sapiens
TAPL
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
32
-
 peptide transport assay Homo sapiens