Literature summary for 7.4.2.5 extracted from

Mori, H.; Ito, K.
The long alpha-helix of SecA is important for the ATPase coupling of translocation (2006), J. Biol. Chem., 281, 36249-36256.

Cloned(Commentary)

Cloned (Comment)	Organism
different plasmids are used, Ara and Lac promoter controlled	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
Cys-less	mutant for analyzing the interaction and regulatory domains of SecA	Escherichia coli
E400C	mutant for analyzing the interaction and regulatory domains of SecA	Escherichia coli
E400C/R642C	mutant for analyzing the interaction and regulatory domains of SecA	Escherichia coli
E400R	mutant for analyzing the interaction and regulatory domains of SecA	Escherichia coli
E400R/A628T	mutant for analyzing the interaction and regulatory domains of SecA	Escherichia coli
E400R/E619K	mutant for analyzing the interaction and regulatory domains of SecA	Escherichia coli
E400R/H620P	mutant for analyzing the interaction and regulatory domains of SecA	Escherichia coli
E400R/I627T	mutant for analyzing the interaction and regulatory domains of SecA	Escherichia coli
E400R/L610P	mutant for analyzing the interaction and regulatory domains of SecA	Escherichia coli
E400R/M607T	mutant for analyzing the interaction and regulatory domains of SecA	Escherichia coli
E400R/N629D	mutant for analyzing the interaction and regulatory domains of SecA	Escherichia coli
E635C	mutant for analyzing the interaction and regulatory domains of SecA	Escherichia coli
R400R/M607T	mutant for analyzing the interaction and regulatory domains of SecA	Escherichia coli
R642C	mutant for analyzing the interaction and regulatory domains of SecA	Escherichia coli
R642E	mutant for analyzing the interaction and regulatory domains of SecA	Escherichia coli
R642E/A628T	mutant for analyzing the interaction and regulatory domains of SecA	Escherichia coli
R642E/E619K	mutant for analyzing the interaction and regulatory domains of SecA	Escherichia coli
R642E/H620P	mutant for analyzing the interaction and regulatory domains of SecA	Escherichia coli
R642E/I627T	mutant for analyzing the interaction and regulatory domains of SecA	Escherichia coli
R642E/L610P	mutant for analyzing the interaction and regulatory domains of SecA	Escherichia coli
R642E/M607T	mutant for analyzing the interaction and regulatory domains of SecA	Escherichia coli
R642E/N629D	mutant for analyzing the interaction and regulatory domains of SecA	Escherichia coli
R656C	mutant for analyzing the interaction and regulatory domains of SecA	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasmic membrane	-	Escherichia coli	-	-
cytosol	-	Escherichia coli	5829	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O	Escherichia coli	-	ADP + phosphate	-	ir

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P10408	-	-

Purification (Commentary)

Purification (Comment)	Organism
inverted membrane vesicles are prepared	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O	-	Escherichia coli	ADP + phosphate	-	ir

Synonyms

Synonyms	Comment	Organism
SecA	-	Escherichia coli
SecA ATPase	-	Escherichia coli