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Literature summary for 7.4.2.4 extracted from

  • Chen, K.; Chen, X.; Schnell, D.J.
    Mechanism of protein import across the chloroplast envelope (2000), Biochem. Soc. Trans., 28, 485-491.
    View publication on PubMed
Search for more literature for 7.4.2.4

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Pisum sativum 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 x * 34000, Toc34, + x * 75000, Toc75 + x * 159000, Toc159, Toc159 is originally identified as an 86000 Da polypeptide, designated Toc86, but it is shown that Toc86 is a proteolytic fragment of the native 159000 protein, the Toc components associate with three inner-membrane proteins, Tic110, Tic22 and Tic20, in a Toc-Tic supercomplex to form functional import sites Pisum sativum
75000
-
 x * 34000, Toc34, + x * 75000, Toc75 + x * 159000, Toc159, Toc159 is originally identified as an 86000 Da polypeptide, designated Toc86, but it is shown that Toc86 is a proteolytic fragment of the native 159000 protein, the Toc components associate with three inner-membrane proteins, Tic110, Tic22 and Tic20, in a Toc-Tic supercomplex to form functional import sites Pisum sativum
159000
-
 x * 34000, Toc34, + x * 75000, Toc75 + x * 159000, Toc159, Toc159 is originally identified as an 86000 Da polypeptide, designated Toc86, but it is shown that Toc86 is a proteolytic fragment of the native 159000 protein, the Toc components associate with three inner-membrane proteins, Tic110, Tic22 and Tic20, in a Toc-Tic supercomplex to form functional import sites Pisum sativum

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O GTP requirement of the Toc complex is attributed to the GTPase component of the complex Pisum sativum ADP + phosphate
-
 ?
ATP + H2O two distinct protein translocation machineries at the outer and inner chloroplast envelope membrane participate in the import of precursor proteins. The translocon at the outer envelope membrane of the chloroplast, Toc, mediates the initial binding of the cytosolic precursor proteins at the chloroplast surface and their subsequent ATP/GTP-dependent translocation across the outer membrane. Protein translocation across the inner membrane is mediated by the translocon at the inner envelope membrane of the chloroplast, Tic, and is driven by ATP hydrolysis in the stroma Pisum sativum ADP + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 34000, Toc34, + x * 75000, Toc75 + x * 159000, Toc159, Toc159 is originally identified as an 86000 Da polypeptide, designated Toc86, but it is shown that Toc86 is a proteolytic fragment of the native 159000 protein, the Toc components associate with three inner-membrane proteins, Tic110, Tic22 and Tic20, in a Toc-Tic supercomplex to form functional import sites Pisum sativum