Literature summary for 7.4.2.3 extracted from

Yamano, K.; Kuroyanagi-Hasegawa, M.; Esaki, M.; Yokota, M.; Endo, T.
Step-size analyses of the mitochondrial Hsp70 import motor reveal the Brownian ratchet in operation (2008), J. Biol. Chem., 283, 27325-27332.

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Protein Variants

Protein Variants	Comment	Organism
additional information	construction of fusion proteins consisting of a mitochondrial presequence containing the first mtHsp70 binding site, a spacer sequence containing an Hsp70 avoidance segment followed by the second mtHsp70 binding site, and different folded mature domains. Analysis of the dependence of the import rates of those fusion proteins on the lengths of Hsp70 avoidance segments, overview. Import of radiolabeled fusion proteins into isolated Saccharomyces cerevisiae mitochondria	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	the presequence-cleaved mature forms of the recombinant fusion proteins with a 50 or 60 residue spacer sequence are proteinase K-resistant, whereas those with a 70 or 80 residue spacer sequence are proteinase K-sensitive	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	in vitro import of the fusion proteins depends on Gly repeat lengths and folded mature domains. Presequence and Gly repeat function as an mtHsp70 binding site and mtHsp70 avoidance segment, respectively	Homo sapiens	5739	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O	Homo sapiens	-	ADP + phosphate	-	?
additional information	Homo sapiens	newly synthesized mitochondrial precursor proteins have to become unfolded by the mitochondrial Hsp70 import motor to cross the mitochondrial membranes, unfolding mechanism, overview	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O	-	Homo sapiens	ADP + phosphate	-	?
additional information	newly synthesized mitochondrial precursor proteins have to become unfolded by the mitochondrial Hsp70 import motor to cross the mitochondrial membranes, unfolding mechanism, overview	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
mitochondrial hsp70	-	Homo sapiens
mtHSP70	-	Homo sapiens

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Release 2023.1 (January 2023)