Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DnaJ | bacterial co-chaperone stimulates ATPase activity of mtHsp70 5fold, that of DnaK 20fold | Saccharomyces cerevisiae | |
fluorescein-CALLQSRLLLSAPRRAAATARY | similar affininty of mtHsp70 and Dnak at 25°C | Saccharomyces cerevisiae | |
GrpE | bacterial co-chaperone stimulates ATPase activity of mtHsp70 5fold, that of DnaK 20fold | Saccharomyces cerevisiae | |
KCCC | comparable stimulation of ATPase activity of mtHsp70 and Dnak | Saccharomyces cerevisiae | |
KKCC | comparable stimulation of ATPase activity of mtHsp70 and Dnak | Saccharomyces cerevisiae | |
Mdj1p | mitochondrial co-chaperone stimulates ATPase activity of both mtHsp70 and Dnak 5-6fold, 20fold activation can be achieved by different molar ratios | Saccharomyces cerevisiae | |
Mge1p | mitochondrial co-chaperone stimulates ATPase activity of both mtHsp70 and Dnak 5-6fold, 20fold activation can be achieved by different molar ratios | Saccharomyces cerevisiae | |
NRLLLTG | substrate binding allosterically stimulates ATPase activity of mtHsp70 2.5 fold, that of Dnak 5fold | Saccharomyces cerevisiae |
Application | Comment | Organism |
---|---|---|
additional information | mtHsp70 and Escherichia coli DnaK display different conformational and biochemical properties, chimeric Hsp70s can not complement DnaK function in vivo | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
chimeric Hsp70s overexpressed in BB1553 cells, mtHsp70 overexpressed in the yeast strain YKN3B | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
inner membrane | - |
Saccharomyces cerevisiae | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
SDS-PAGE, possibly the ATPase domain of mtHsp70 | Saccharomyces cerevisiae |
58000 | - |
SDS-PAGE, fragment in the presence of ATP | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by ion exchange, ATP-agarose affinity and hydroxyapatite chromatographies | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | activity of mtHsp70 higher than that of DnaK | Saccharomyces cerevisiae | ADP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
mitochondrial hsp70 | - |
Saccharomyces cerevisiae |
mtHSP70 | - |
Saccharomyces cerevisiae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 76.2 | mtHsp70 binding kinetics are faster at higher temperatures | Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
51.6 | - |
thermal denaturation of mtHsp70 shows three endotherms centered at 51.6, 67.5 and 76.2°C, the endotherm at 51.6°C may represent the unfolding of a more stable ATPase domain | Saccharomyces cerevisiae |