Literature summary for 7.4.2.11 extracted from

Nguyen, P.T.; Lai, J.Y.; Lee, A.T.; Kaiser, J.T.; Rees, D.C.
Noncanonical role for the binding protein in substrate uptake by the MetNI methionine ATP binding cassette (ABC) transporter (2018), Proc. Natl. Acad. Sci. USA, 115, 10596-10604 .

Search for more literature for 7.4.2.11

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21-Gold (DE3) cells	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
ATPgammaS-bound MetNIQ complex in the outwardfacing conformation with the N229A apo MetQ variant, hanging drop vapor diffusion method, using 0.1 M Mes, pH 6.0, and 22% (w/v) PEG 400	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
N229A	the substrate-binding deficient variant of the cognate binding protein MetQ supports high transport activity toward D-selenomethionine	Escherichia coli
N295A	the mutation results in a higher maximal uptake rate with no change in Km value compared to the wild type enzyme	Escherichia coli
N295A/F103A	inactive	Escherichia coli
N295A/M163A	the mutations result in a lower maximal uptake rate with decreased Km value compared to the wild type enzyme	Escherichia coli
N295A/N229A	the mutations result in a higher maximal uptake rate with increased Km value compared to the wild type enzyme	Escherichia coli
N295A/Y160A	inactive	Escherichia coli
N295A/Y160A	the mutations result in a higher maximal uptake rate with increased Km value compared to the wild type enzyme	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0014	-	D-selenomethionine-[methionine-binding protein][side 1]	mutant enzyme N295A/M163A, at pH 6.5 and 37°C	Escherichia coli
0.0017	-	D-selenomethionine-[methionine-binding protein][side 1]	mutant enzyme N295A, at pH 6.5 and 37°C	Escherichia coli
0.0018	-	D-selenomethionine-[methionine-binding protein][side 1]	wild type enzyme, at pH 6.5 and 37°C	Escherichia coli
0.0041	-	D-selenomethionine-[methionine-binding protein][side 1]	mutant enzyme N229A/Y160A, at pH 6.5 and 37°C	Escherichia coli
0.0048	-	D-selenomethionine-[methionine-binding protein][side 1]	mutant enzyme N295A/N229A, at pH 6.5 and 37°C	Escherichia coli
0.0074	-	D-selenomethionine-[methionine-binding protein][side 1]	mutant enzyme N229A, at pH 6.5 and 37°C	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Escherichia coli	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + D-methionine-[methionine-binding protein][side 1]	Escherichia coli	-	ADP + phosphate + D-methionine[side 2] + [methionine-binding protein][side 1]	-	?
ATP + H2O + L-methionine-[methionine-binding protein][side 1]	Escherichia coli	-	ADP + phosphate + L-methionine[side 2] + [methionine-binding protein][side 1]	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P30750 and P31547	metN subunit and metI subunit	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-Sepharose column chromatography and gel filtration	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + D-methionine-[methionine-binding protein][side 1]	-	Escherichia coli	ADP + phosphate + D-methionine[side 2] + [methionine-binding protein][side 1]	-	?
ATP + H2O + D-selenomethionine-[methionine-binding protein][side 1]	-	Escherichia coli	ADP + phosphate + D-selenomethionine[side 2] + [methionine-binding protein][side 1]	-	?
ATP + H2O + L-methionine-[methionine-binding protein][side 1]	-	Escherichia coli	ADP + phosphate + L-methionine[side 2] + [methionine-binding protein][side 1]	-	?

Synonyms

Synonyms	Comment	Organism
methionine ATP binding cassette transporter	-	Escherichia coli
MetNI	-	Escherichia coli

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Release 2023.1 (January 2023)