Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 7.4.2.1 extracted from

  • Svennerstam, H.; Jaemtgard, S.; Ahmad, I.; Huss-Danell, K.; Naesholm, T.; Ganeteg, U.
    Transporters in Arabidopsis roots mediating uptake of amino acids at naturally occurring concentrations (2011), New Phytol., 191, 459-467.
    View publication on PubMed
Search for more literature for 7.4.2.1

Protein Variants

Protein Variants Comment Organism
additional information overexpression of LHT1 in Arabidopsis thaliana results in increased uptake, with uptake rates of between 219 and 456% of wild-type uptake, and saturating kinetics for L-Gln and increased but linear kinetics for L-Ala, L-Asp and L-Glu, in comparison to the wild-type plant. In the LHT1 mutant, uptake of L-Gln, L-Ala, L-Glu and L-Asp is greatly reduced by on average 61-85% over the entire concentration range, while the uptake of L-Arg and L-Lys is unaffected. AAP1 mutants do not display any major differences in the uptake of any of the amino acids tested Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.041
-
 L-glutamine/out wild-type LHT1, pH 5.8, temperature not specified in the publication Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Arabidopsis thaliana Col-0
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Arabidopsis thaliana
-
shoot
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + L-alanine/out
-
 Arabidopsis thaliana ADP + phosphate + L-alanine/in
-
 ?
ATP + H2O + L-alanine/out
-
 Arabidopsis thaliana Col-0 ADP + phosphate + L-alanine/in
-
 ?
ATP + H2O + L-aspartate/out
-
 Arabidopsis thaliana ADP + phosphate + L-aspartate/in
-
 ?
ATP + H2O + L-aspartate/out
-
 Arabidopsis thaliana Col-0 ADP + phosphate + L-aspartate/in
-
 ?
ATP + H2O + L-glutamate/out
-
 Arabidopsis thaliana ADP + phosphate + L-glutamate/in
-
 ?
ATP + H2O + L-glutamate/out
-
 Arabidopsis thaliana Col-0 ADP + phosphate + L-glutamate/in
-
 ?
ATP + H2O + L-glutamine/out
-
 Arabidopsis thaliana ADP + phosphate + glutamine/in
-
 ?
ATP + H2O + L-histidine/out
-
 Arabidopsis thaliana ADP + phosphate + L-histidine/in
-
 ?
ATP + H2O + L-histidine/out
-
 Arabidopsis thaliana Col-0 ADP + phosphate + L-histidine/in
-
 ?
ATP + H2O + neutral amino acid/out
-
 Arabidopsis thaliana ADP + phosphate + neutral amino acid/in
-
 ?
additional information LHT1 displays high affinity for neutral amino acids, L-His, and acidic amino acids. Amino acid permease 1 mediates uptake of several neutral amino acids as well as L-Glu and L-His but, similar to LHT1, activity for L-Arg and L-Lys is not detected Arabidopsis thaliana ?
-
 ?
additional information LHT1 displays high affinity for neutral amino acids, L-His, and acidic amino acids. Amino acid permease 1 mediates uptake of several neutral amino acids as well as L-Glu and L-His but, similar to LHT1, activity for L-Arg and L-Lys is not detected Arabidopsis thaliana Col-0 ?
-
 ?

Synonyms

Synonyms Comment Organism
AAP1
-
 Arabidopsis thaliana
amino acid permease 1
-
 Arabidopsis thaliana
LHT1
-
 Arabidopsis thaliana
lysine histidine transporter 1
-
 Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
 assay at Arabidopsis thaliana