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Literature summary for 7.4.2.1 extracted from

  • Inbar, E.; Canepa, G.E.; Carrillo, C.; Glaser, F.; Suter Grotemeyer, M.; Rentsch, D.; Zilberstein, D.; Pereira, C.A.
    Lysine transporters in human trypanosomatid pathogens (2012), Amino Acids, 42, 347-360.
    View publication on PubMed
Search for more literature for 7.4.2.1

Activating Compound

Activating Compound Comment Organism Structure
L-asparagine slight activation Trypanosoma cruzi
L-cysteine slight activation Leishmania donovani
L-threonine slight activation Leishmania donovani

Cloned(Commentary)

Cloned (Comment) Organism
gene LdAAP7, phylogenetic analysis, functional expression in enzyme-deficient Saccharomyces cerevisiae mutant strain 22DELTA7AA Leishmania donovani
gene TcAAP7, phylogenetic analysis, functional expression in enzyme-deficient Saccharomyces cerevisiae mutant strain 22DELTA7AA. Functional overexpression of GFP-tagged TcAAP7 Trypanosoma cruzi

Inhibitors

Inhibitors Comment Organism Structure
glycine
-
 Trypanosoma cruzi
L-aspartate slight inhibition Leishmania donovani
L-cysteine
-
 Trypanosoma cruzi
L-histidine slight inhibition Trypanosoma cruzi
L-methionine
-
 Trypanosoma cruzi
L-threonine
-
 Trypanosoma cruzi
additional information no or poor inhibition by L-alanine, L-asparagine, L-arginine, L-proline, L-serine, glycine, L-methionine, L-threonine, L-histidine, and L-cysteine Leishmania donovani
additional information no inhibition by L-aspartate, L-arginine, L-asparagine, L-alanine, and L-serine Trypanosoma cruzi
proline
-
 Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00736
-
 L-lysine/out pH 4.5, 30°C, recombinant enzyme Leishmania donovani

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface
-
 Leishmania donovani 9986
-
flagellum
-
 Leishmania donovani
-
-
membrane
-
 Leishmania donovani 16020
-
membrane mainly to a membrane-bound structure or invagination close to the kinetoplast, the latter corresponding to the flagellar pocket or associated structures, such as the cytostome or contractile vacuole Trypanosoma cruzi 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + L-lysine/out Leishmania donovani
-
 ADP + phosphate + L-lysine/in
-
 ?
ATP + H2O + L-lysine/out Trypanosoma cruzi
-
 ADP + phosphate + L-lysine/in
-
 ?
ATP + H2O + polar amino acid/out Leishmania donovani
-
 ADP + phosphate + polar amino acid/in
-
 ?
ATP + H2O + polar amino acid/out Trypanosoma cruzi
-
 ADP + phosphate + polar amino acid/in
-
 ?

Organism

Organism UniProt Comment Textmining
Leishmania donovani Q208R5 gene LdAAP7
-
Trypanosoma cruzi Q4DMS3 gene TcAAP7
-

Source Tissue

Source Tissue Comment Organism Textmining
epimastigote
-
 Trypanosoma cruzi
-
promastigote
-
 Leishmania donovani
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + L-lysine/out
-
 Leishmania donovani ADP + phosphate + L-lysine/in
-
 ?
ATP + H2O + L-lysine/out
-
 Trypanosoma cruzi ADP + phosphate + L-lysine/in
-
 ?
ATP + H2O + polar amino acid/out
-
 Leishmania donovani ADP + phosphate + polar amino acid/in
-
 ?
ATP + H2O + polar amino acid/out
-
 Trypanosoma cruzi ADP + phosphate + polar amino acid/in
-
 ?
additional information specificity of L-lysine transport in wild-type cells and in recombinantly parasite AAP7 expressing yeast 22DELTA7AA cells, overview Leishmania donovani ?
-
 ?
additional information specificity of L-lysine transport in wild-type cells, overview Trypanosoma cruzi ?
-
 ?

Synonyms

Synonyms Comment Organism
AAP7
-
 Leishmania donovani
AAP7
-
 Trypanosoma cruzi
amino acid permease 7
-
 Leishmania donovani
lysine permease
-
 Leishmania donovani
lysine permease
-
 Trypanosoma cruzi
lysine-specific permease
-
 Leishmania donovani
lysine-specific permease
-
 Trypanosoma cruzi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
 assay at Trypanosoma cruzi
30
-
 assay at Leishmania donovani

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 recombinant enzyme Leishmania donovani
7
-
 assay at Trypanosoma cruzi

General Information

General Information Comment Organism
evolution the enzyme belongs to the large amino acid/auxin permease family Leishmania donovani