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Literature summary for 7.3.2.6 extracted from

  • Otrelo-Cardoso, A.R.; Nair, R.R.; Correia, M.A.S.; Cordeiro, R.S.C.; Panjkovich, A.; Svergun, D.I.; Santos-Silva, T.; Rivas, M.G.
    Highly selective tungstate transporter protein TupA from Desulfovibrio alaskensis G20 (2017), Sci. Rep., 7, 5798 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.3.2.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Tuner (DE3) cells Oleidesulfovibrio alaskensis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.2 M magnesium chloride, 0.1 M HEPES (pH 7.5), and 30% (w/v) polyethylene glycol 3350 Oleidesulfovibrio alaskensis

Protein Variants

Protein Variants Comment Organism
R118E the mutation prevents the binding of the enzyme to both molybdate and tungstate Oleidesulfovibrio alaskensis
R118K the mutant loses the ability to coordinate molybdate and keeps an extremely high affinity and selectivity for tungstate Oleidesulfovibrio alaskensis
R118Q the mutant loses the ability to coordinate molybdate and binds tungstate with much less affinity than the wild type enzyme Oleidesulfovibrio alaskensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + molybdate-[molybdate-binding protein][side 1] Oleidesulfovibrio alaskensis
-
 ADP + phosphate + molybdate[side 2] + [molybdate-binding protein][side 1]
-
 ?
ATP + H2O + molybdate-[molybdate-binding protein][side 1] Oleidesulfovibrio alaskensis G20
-
 ADP + phosphate + molybdate[side 2] + [molybdate-binding protein][side 1]
-
 ?
ATP + H2O + tungstate-[tungstate-binding protein][side 1] Oleidesulfovibrio alaskensis
-
 ADP + phosphate + tungstate[side 2] + [tungstate-binding protein][side 1]
-
 ?
ATP + H2O + tungstate-[tungstate-binding protein][side 1] Oleidesulfovibrio alaskensis G20
-
 ADP + phosphate + tungstate[side 2] + [tungstate-binding protein][side 1]
-
 ?

Organism

Organism UniProt Comment Textmining
Oleidesulfovibrio alaskensis Q316W1
-
-
Oleidesulfovibrio alaskensis G20 Q316W1
-
-

Purification (Commentary)

Purification (Comment) Organism
His GraviTrap column chromatography Oleidesulfovibrio alaskensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + molybdate-[molybdate-binding protein][side 1]
-
 Oleidesulfovibrio alaskensis ADP + phosphate + molybdate[side 2] + [molybdate-binding protein][side 1]
-
 ?
ATP + H2O + molybdate-[molybdate-binding protein][side 1]
-
 Oleidesulfovibrio alaskensis G20 ADP + phosphate + molybdate[side 2] + [molybdate-binding protein][side 1]
-
 ?
ATP + H2O + tungstate-[tungstate-binding protein][side 1]
-
 Oleidesulfovibrio alaskensis ADP + phosphate + tungstate[side 2] + [tungstate-binding protein][side 1]
-
 ?
ATP + H2O + tungstate-[tungstate-binding protein][side 1]
-
 Oleidesulfovibrio alaskensis G20 ADP + phosphate + tungstate[side 2] + [tungstate-binding protein][side 1]
-
 ?
additional information no activity with SO42-, PO43- and ClO4- Oleidesulfovibrio alaskensis ?
-
 ?
additional information no activity with SO42-, PO43- and ClO4- Oleidesulfovibrio alaskensis G20 ?
-
 ?

Synonyms

Synonyms Comment Organism
tungstate transporter
-
 Oleidesulfovibrio alaskensis
TupA
-
 Oleidesulfovibrio alaskensis