Literature summary for 7.3.2.6 extracted from

Otrelo-Cardoso, A.R.; Nair, R.R.; Correia, M.A.; Rivas, M.G.; Santos-Silva, T.
TupA: a tungstate binding protein in the periplasm of Desulfovibrio alaskensis G20 (2014), Int. J. Mol. Sci., 15, 11783-11798.

Search for more literature for 7.3.2.6

Cloned(Commentary)

Cloned (Comment)	Organism
gene tupA, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of the soluble TupA component in Escherichia coli strain BL21(DE3)	Oleidesulfovibrio alaskensis

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant component TupA, hanging drop vapor diffusion method, mixing of 0.002 ml of 7.5 mg/ml protein in 5 mM Tris-HCl, pH 7.5, with 0.001 ml of reservoir solution containing 0.2 M magnesium chloride, 0.1 M HEPES, pH 7.5, and 30% w/v PEG 3350, 20°C, 4 days, X-ray diffraction structure determination and analysis at 1.4 A resolution, molecular replacement	Oleidesulfovibrio alaskensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	component TupA has higher affinity to tungstate than molybdate, oxoanion binding kinetic of TupA, overview	Oleidesulfovibrio alaskensis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Oleidesulfovibrio alaskensis	16020	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
29000	-	x * 29000, recombinant enzyme, SDS-PAGE	Oleidesulfovibrio alaskensis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + tungstate[side 1]	Oleidesulfovibrio alaskensis	-	ADP + phosphate + tungstate[side 2]	-	?
ATP + H2O + tungstate[side 1]	Oleidesulfovibrio alaskensis G20	-	ADP + phosphate + tungstate[side 2]	-	?

Organism

Organism	UniProt	Comment	Textmining
Oleidesulfovibrio alaskensis	Q316W1	component TupA; gene Dde_0234 or tupA encoding component TupA, organized in the gene cluster tupABC	-
Oleidesulfovibrio alaskensis G20	Q316W1	component TupA; gene Dde_0234 or tupA encoding component TupA, organized in the gene cluster tupABC	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant soluble component TupA from Escherichia coli strain BL21 (DE3) by anion exchange chromatography, gel filtration, and ultrafiltration	Oleidesulfovibrio alaskensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + tungstate[side 1]	-	Oleidesulfovibrio alaskensis	ADP + phosphate + tungstate[side 2]	-	?
ATP + H2O + tungstate[side 1]	-	Oleidesulfovibrio alaskensis G20	ADP + phosphate + tungstate[side 2]	-	?
additional information	component TupA binds both tungstate and molybdate ions and has no significant interaction with sulfate, phosphate or perchlorate, quantitative analysis of metal binding by isothermal titration calorimetry, overview	Oleidesulfovibrio alaskensis	?	-	?
additional information	component TupA binds both tungstate and molybdate ions and has no significant interaction with sulfate, phosphate or perchlorate, quantitative analysis of metal binding by isothermal titration calorimetry, overview	Oleidesulfovibrio alaskensis G20	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 29000, recombinant enzyme, SDS-PAGE	Oleidesulfovibrio alaskensis

Synonyms

Synonyms	Comment	Organism
TupABC	-	Oleidesulfovibrio alaskensis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Oleidesulfovibrio alaskensis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Oleidesulfovibrio alaskensis

pI Value

Organism	Comment	pI Value Maximum	pI Value
Oleidesulfovibrio alaskensis	isoelectric focusing	-	5.69

Expression

Organism	Comment	Expression
Oleidesulfovibrio alaskensis	the protein involved in tungstate transport (TupA) is downregulated under high molybdate concentration stress conditions	down

General Information

General Information	Comment	Organism
physiological function	the TupABC system is involved in the cellular uptake of tungsten and belongs to the ABC (ATP binding cassette)-type transporter systems. The TupA component is a periplasmic protein that binds tungstate anions, which are then transported through the membrane by the TupB component using ATP hydrolysis as the energy source, the reaction is catalyzed by the TupC component. The genes encoding the three components are organized in an operon wtpABC or gene cluster (tupABC) regulated by a transcription factor. Under oxoanion starvation, the component A binds tungstate and interacts with the component B to actively transport tungstate from the periplasm to the cytoplasm. The TupABC transport system and, more specifically, the component A may constitute the first selection gate from which cells differentiate between Mo and W. TupA is a tungstate-binding protein that is able to bind tungstate and molybdate ions. Regulation of the transport enzyme complex, overview	Oleidesulfovibrio alaskensis

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Release 2023.1 (January 2023)