Cloned (Comment) | Organism |
---|---|
gene cj1540 or tupA, overexpression in Campylobacter jejuni strain NCTC 11168, overexpression with TupA native N-terminal signal sequence results in only the mature periplasmic form accumulates, which is highly soluble and stable | Campylobacter jejuni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | ligand binding analysis by mass spectrometry and isothermal titration calorimetry, thermodynamics and ion dissociation kinetics, overview. Tungstate binds 50 000fold more tightly than molybdate to Cj1540 | Campylobacter jejuni |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Campylobacter jejuni | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + tungstate/out | Campylobacter jejuni | the enzyme is part of the the Cj1538-1540 system | ADP + phosphate + tungstate/in | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylobacter jejuni | Q0P885 | gene Cj1540; gene cj1540 or tupA | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | Cj1540 contains a simpler N-terminal signal sequence with a single signal peptidase I cleavage site | Campylobacter jejuni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + tungstate/out | the enzyme is part of the the Cj1538-1540 system | Campylobacter jejuni | ADP + phosphate + tungstate/in | - |
? | |
ATP + H2O + tungstate/out | tungstate binds 50000fold more tightly than molybdate to Cj1540 | Campylobacter jejuni | ADP + phosphate + tungstate/in | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | Cj1540 contains four tryptophans and seven tyrosines in the mature protein | Campylobacter jejuni |
Synonyms | Comment | Organism |
---|---|---|
Cj1540 | - |
Campylobacter jejuni |
tungstate transporter | - |
Campylobacter jejuni |
TupA | - |
Campylobacter jejuni |
TupABC-like ultra-high affinity tungstate transporter | - |
Campylobacter jejuni |
General Information | Comment | Organism |
---|---|---|
metabolism | Campylobacter jejuni possesses a specific, ultra high affinity tungstate transporter that supplies tungsten for incorporation into formate dehydrogenase, in additon to a molybdate/tungstate transporter ModA, EC 3.6.3.29, and also two MoeA paralogues which may explain the formation of both molybdopterin and tungstopterin in this bacterium | Campylobacter jejuni |
additional information | cj1540 (tupA) mutant shows a low cellular tungsten content, and a decrase in formate dehydrogenase activity by about 50% in the tupA strain. Supplementation of the growth medium with tungstate significantly increases formate dehydrogenase activity in the wild-type, while inhibiting known molybdoenzymes | Campylobacter jejuni |
physiological function | Campylobacter jejuni possesses a specific, ultra high affinity tungstate transporter that supplies tungsten for incorporation into formate dehydrogenase, in additon to a molybdate/tungstate transporter ModA, EC 3.6.3.29 | Campylobacter jejuni |