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Literature summary for 7.3.2.6 extracted from

  • Smart, J.P.; Cliff, M.J.; Kelly, D.J.
    A role for tungsten in the biology of Campylobacter jejuni: tungstate stimulates formate dehydrogenase activity and is transported via an ultra-high affinity ABC system distinct from the molybdate transporter (2009), Mol. Microbiol., 74, 742-757.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene cj1540 or tupA, overexpression in Campylobacter jejuni strain NCTC 11168, overexpression with TupA native N-terminal signal sequence results in only the mature periplasmic form accumulates, which is highly soluble and stable Campylobacter jejuni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information ligand binding analysis by mass spectrometry and isothermal titration calorimetry, thermodynamics and ion dissociation kinetics, overview. Tungstate binds 50 000fold more tightly than molybdate to Cj1540 Campylobacter jejuni

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Campylobacter jejuni
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + tungstate/out Campylobacter jejuni the enzyme is part of the the Cj1538-1540 system ADP + phosphate + tungstate/in
-
?

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni Q0P885 gene Cj1540; gene cj1540 or tupA
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information Cj1540 contains a simpler N-terminal signal sequence with a single signal peptidase I cleavage site Campylobacter jejuni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + tungstate/out the enzyme is part of the the Cj1538-1540 system Campylobacter jejuni ADP + phosphate + tungstate/in
-
?
ATP + H2O + tungstate/out tungstate binds 50000fold more tightly than molybdate to Cj1540 Campylobacter jejuni ADP + phosphate + tungstate/in
-
?

Subunits

Subunits Comment Organism
More Cj1540 contains four tryptophans and seven tyrosines in the mature protein Campylobacter jejuni

Synonyms

Synonyms Comment Organism
Cj1540
-
Campylobacter jejuni
tungstate transporter
-
Campylobacter jejuni
TupA
-
Campylobacter jejuni
TupABC-like ultra-high affinity tungstate transporter
-
Campylobacter jejuni

General Information

General Information Comment Organism
metabolism Campylobacter jejuni possesses a specific, ultra high affinity tungstate transporter that supplies tungsten for incorporation into formate dehydrogenase, in additon to a molybdate/tungstate transporter ModA, EC 3.6.3.29, and also two MoeA paralogues which may explain the formation of both molybdopterin and tungstopterin in this bacterium Campylobacter jejuni
additional information cj1540 (tupA) mutant shows a low cellular tungsten content, and a decrase in formate dehydrogenase activity by about 50% in the tupA strain. Supplementation of the growth medium with tungstate significantly increases formate dehydrogenase activity in the wild-type, while inhibiting known molybdoenzymes Campylobacter jejuni
physiological function Campylobacter jejuni possesses a specific, ultra high affinity tungstate transporter that supplies tungsten for incorporation into formate dehydrogenase, in additon to a molybdate/tungstate transporter ModA, EC 3.6.3.29 Campylobacter jejuni