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Literature summary for 7.3.2.6 extracted from

  • Makdessi, K.; Andreesen, J.R.; Pich, A.
    Tungstate uptake by a highly specific ABC transporter in Eubacterium acidaminophilum (2001), J. Biol. Chem., 276, 24557-24564.
    View publication on PubMed
Search for more literature for 7.3.2.6

Cloned(Commentary)

Cloned (Comment) Organism
genes tupABC, DNA and amino acid sequence determination and analysis, genetic organization and transcriptional analysis of the sequenced tup genes and the downstream region, phylogenetic analysis and tree, overexpression of the substrate-binding protein of the transporter, TupA, in Escherichia coli Peptoclostridium acidaminophilum

Inhibitors

Inhibitors Comment Organism Structure
additional information no impairment of tungstate uptake is observed if the ATPase inhibitor arsenate or the uncoupler 2,4-dinitrophenol are present during the uptake experiments by resting cells Peptoclostridium acidaminophilum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + tungstate/out Peptoclostridium acidaminophilum
-
 ADP + phosphate + tungstate/in
-
 ?
ATP + H2O + tungstate/out Peptoclostridium acidaminophilum DSM 5388
-
 ADP + phosphate + tungstate/in
-
 ?
additional information Peptoclostridium acidaminophilum tungstate uptake by Eubacterium acidaminophilum is not affected by equimolar amounts of molybdate ?
-
 ?
additional information Peptoclostridium acidaminophilum DSM 5388 tungstate uptake by Eubacterium acidaminophilum is not affected by equimolar amounts of molybdate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Peptoclostridium acidaminophilum Q93KD6 tupA; genes tupABC coding for an ABC transporter specific for tungstate
-
Peptoclostridium acidaminophilum DSM 5388 Q93KD6 tupA; genes tupABC coding for an ABC transporter specific for tungstate
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + tungstate/out
-
 Peptoclostridium acidaminophilum ADP + phosphate + tungstate/in
-
 ?
ATP + H2O + tungstate/out only tungstate induces a shift of TupA mobility, suggesting that only this anion is specifically bound by TupA. The TupABC transporter is specific for tungstate Peptoclostridium acidaminophilum ADP + phosphate + tungstate/in
-
 ?
ATP + H2O + tungstate/out
-
 Peptoclostridium acidaminophilum DSM 5388 ADP + phosphate + tungstate/in
-
 ?
ATP + H2O + tungstate/out only tungstate induces a shift of TupA mobility, suggesting that only this anion is specifically bound by TupA. The TupABC transporter is specific for tungstate Peptoclostridium acidaminophilum DSM 5388 ADP + phosphate + tungstate/in
-
 ?
additional information tungstate uptake by Eubacterium acidaminophilum is not affected by equimolar amounts of molybdate Peptoclostridium acidaminophilum ?
-
 ?
additional information tungstate uptake by Eubacterium acidaminophilum is not affected by equimolar amounts of molybdate Peptoclostridium acidaminophilum DSM 5388 ?
-
 ?

Synonyms

Synonyms Comment Organism
More the enzyme belongs to a separate phylogenetic group of oxyanion ABC transporters Peptoclostridium acidaminophilum
tungstate transporter
-
 Peptoclostridium acidaminophilum
TupA
-
 Peptoclostridium acidaminophilum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Peptoclostridium acidaminophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Peptoclostridium acidaminophilum

General Information

General Information Comment Organism
metabolism tupABC gene encoded enzymes participate in the biosynthesis of the pterin cofactor that is present in molybdenum- and tungsten-containing enzymes except nitrogenase Peptoclostridium acidaminophilum