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Literature summary for 7.3.2.4 extracted from

  • Yan, H.; Huang, W.; Yan, C.; Gong, X.; Jiang, S.; Zhao, Y.; Wang, J.; Shi, Y.
    Structure and mechanism of a nitrate transporter (2013), Cell Rep., 3, 716-723.
    View publication on PubMed
Search for more literature for 7.3.2.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method Escherichia coli

Protein Variants

Protein Variants Comment Organism
F145A the mutant displays binding to both nitrate and nitrite Escherichia coli
F265A the mutant retains very weak binding to nitrate but not to nitrite Escherichia coli
F367A the mutation abrogates binding to both nitrate and nitrite Escherichia coli
F47A the mutation abrogates binding to both nitrate and nitrite Escherichia coli
N173A the mutant retains some binding to substrate, albeit drastically reduced in comparison to the wild type Escherichia coli
R303A the mutation completely abrogates binding to nitrate or nitrite Escherichia coli
R87A the mutation completely abrogates binding to nitrate or nitrite Escherichia coli
W50A the mutation abrogates binding to both nitrate and nitrite Escherichia coli
Y261A, the mutation completely abrogates binding to nitrate or nitrite Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + nitrate/out Escherichia coli
-
 ADP + phosphate + nitrate/in
-
 ?
ATP + H2O + nitrite/out Escherichia coli
-
 ADP + phosphate + nitrite/in
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P37758
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + nitrate/out
-
 Escherichia coli ADP + phosphate + nitrate/in
-
 ?
ATP + H2O + nitrite/out
-
 Escherichia coli ADP + phosphate + nitrite/in
-
 ?

Synonyms

Synonyms Comment Organism
NarU
-
 Escherichia coli
nitrate/nitrite transporter
-
 Escherichia coli