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Literature summary for 7.3.2.4 extracted from

  • Morere-Le Paven, M.C.; Viau, L.; Hamon, A.; Vandecasteele, C.; Pellizzaro, A.; Bourdin, C.; Laffont, C.; Lapied, B.; Lepetit, M.; Frugier, F.; Legros, C.; Limami, A.M.
    Characterization of a dual-affinity nitrate transporter MtNRT1.3 in the model legume Medicago truncatula (2011), J. Exp. Bot., 62, 5595-5605.
    View publication on PubMed
Search for more literature for 7.3.2.4

Cloned(Commentary)

Cloned (Comment) Organism
gene Medtr5g093170.1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of NRT1.3 in Xenopus laevis oocytes Medicago truncatula

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0416
-
 nitrate/out recombinant enzyme in frog oocytes, pH 5.5, temperature not specified in the publication Medicago truncatula

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + nitrate/out Medicago truncatula
-
 ADP + phosphate + nitrate/in
-
 ?
ATP + H2O + nitrate/out Medicago truncatula Jemalong-6
-
 ADP + phosphate + nitrate/in
-
 ?
ATP + H2O + nitrate/out Medicago truncatula DZA315.16
-
 ADP + phosphate + nitrate/in
-
 ?

Organism

Organism UniProt Comment Textmining
Medicago truncatula D5LQ68 gene Medtr5g093170.1
-
Medicago truncatula DZA315.16 D5LQ68 gene Medtr5g093170.1
-
Medicago truncatula Jemalong-6 D5LQ68 gene Medtr5g093170.1
-

Source Tissue

Source Tissue Comment Organism Textmining
root MtNRT1.3 expression is developmentally regulated in roots, with increasing expression after completion of germination in N-free medium Medicago truncatula
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + nitrate/out
-
 Medicago truncatula ADP + phosphate + nitrate/in
-
 ?
ATP + H2O + nitrate/out
-
 Medicago truncatula Jemalong-6 ADP + phosphate + nitrate/in
-
 ?
ATP + H2O + nitrate/out
-
 Medicago truncatula DZA315.16 ADP + phosphate + nitrate/in
-
 ?

Synonyms

Synonyms Comment Organism
nitrate transporter
-
 Medicago truncatula
NRT1.3
-
 Medicago truncatula

Expression

Organism Comment Expression
Medicago truncatula MtNRT1.3 is environmentally downregulated by the addition of NO3- to the roots down
Medicago truncatula MtNRT1.3 is environmentally upregulated by the absence of NO3-, not as a result of a systemic signalling of plant N status up

General Information

General Information Comment Organism
evolution the enzyme is a member of the NRT1 superfamily Medicago truncatula
additional information MtNRT1.3 is a dual-affinity NO3- transporter Medicago truncatula
physiological function nitrate transporter NRT1.3 is involved in the control of primary root growth and NO3- sensing acting in the response to N limitation, which increases the ability of the plant to acquire NO3- under N-limiting conditions Medicago truncatula