Cloned (Comment) | Organism |
---|---|
inducible expression of Rv1739c in an Escherichia coli strain EWP44 lacking CysA does not increase sulfate uptake by intact cells. Expression of Rv1739c in a Mycobacterium bovis BCG strain lacking the ABC sulfate permease subunit CysA cannot complement sulfate auxotrophy. Induction of Rv1739c expression in Escherichia coli increases bacterial uptake of sulfate, but not Cl-, formate, or oxalate. Sulfate uptake is also increased by overexpression of the Rv1739c transmembrane domain, but not of the cytoplasmic C-terminal STAS domain | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutation to serine of the three cysteine residues of Rv1739c does not affect magnitude, pH-dependence, or pharmacology of sulfate uptake | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
carbonyl cyanide 3-chloro-phenylhydrazone | - |
Mycobacterium tuberculosis | |
additional information | no inhibition by bicarbonate, nitrate, or phosphate, or by the anion transport inhibitor NS3623 | Mycobacterium tuberculosis | |
N-ethylmaleimide | - |
Mycobacterium tuberculosis | |
selenate | - |
Mycobacterium tuberculosis | |
sulfite | - |
Mycobacterium tuberculosis | |
thiosulfate | - |
Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
sulfate | pH 6.0, recombinant enzyme | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Na+-independent sulfate uptake | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mycobacterium tuberculosis | facilitation of bacterial sulfate uptake by Rv1739c requires CysA and its associated sulfate permease activity, thus Rv1739c may be a CysTWA-dependent sulfate transporter | ? | - |
? | |
additional information | Mycobacterium tuberculosis H37Rv | facilitation of bacterial sulfate uptake by Rv1739c requires CysA and its associated sulfate permease activity, thus Rv1739c may be a CysTWA-dependent sulfate transporter | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
gene Rv1739c | - |
Mycobacterium tuberculosis H37Rv | - |
gene Rv1739c | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + sulfate/out | facilitation of bacterial sulfate uptake by Rv1739c requires CysA and its associated sulfate permease activity, thus Rv1739c may be a CysTWA-dependent sulfate transporter | Mycobacterium tuberculosis | ADP + phosphate + sulfate/in | - |
? | |
ATP + H2O + sulfate/out | facilitation of bacterial sulfate uptake by Rv1739c requires CysA and its associated sulfate permease activity, thus Rv1739c may be a CysTWA-dependent sulfate transporter | Mycobacterium tuberculosis H37Rv | ADP + phosphate + sulfate/in | - |
? | |
additional information | facilitation of bacterial sulfate uptake by Rv1739c requires CysA and its associated sulfate permease activity, thus Rv1739c may be a CysTWA-dependent sulfate transporter | Mycobacterium tuberculosis | ? | - |
? | |
additional information | Na+-independent sulfate uptake | Mycobacterium tuberculosis | ? | - |
? | |
additional information | facilitation of bacterial sulfate uptake by Rv1739c requires CysA and its associated sulfate permease activity, thus Rv1739c may be a CysTWA-dependent sulfate transporter | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
additional information | Na+-independent sulfate uptake | Mycobacterium tuberculosis H37Rv | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Rv1739c | - |
Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Mycobacterium tuberculosis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | no activity below pH 5.0 and above pH 8.0 | Mycobacterium tuberculosis |