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show all sequences of 7.3.2.3

Structure of the ATPase subunit CysA of the putative sulfate ATP-binding cassette (ABC) transporter from Alicyclobacillus acidocaldarius

Scheffel, F.; Demmer, U.; Warkentin, E.; Huelsmann, A.; Schneider, E.; Ermler, U.; FEBS Lett. 579, 2953-2958 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli strain JM109
Alicyclobacillus acidocaldarius
Crystallization (Commentary)
Crystallization
Organism
hanging drop vapour diffusion method
Alicyclobacillus acidocaldarius
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4
-
ATP
wild type enzyme CysA, at 60C
Alicyclobacillus acidocaldarius
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Alicyclobacillus acidocaldarius
-
-
-
Purification (Commentary)
Commentary
Organism
Ni-NTA column chromatography
Alicyclobacillus acidocaldarius
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + H2O + sulfate/out
-
668610
Alicyclobacillus acidocaldarius
ADP + phosphate + sulfate/in
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
x-ray crystallography
Alicyclobacillus acidocaldarius
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli strain JM109
Alicyclobacillus acidocaldarius
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging drop vapour diffusion method
Alicyclobacillus acidocaldarius
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4
-
ATP
wild type enzyme CysA, at 60C
Alicyclobacillus acidocaldarius
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography
Alicyclobacillus acidocaldarius
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + H2O + sulfate/out
-
668610
Alicyclobacillus acidocaldarius
ADP + phosphate + sulfate/in
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
x-ray crystallography
Alicyclobacillus acidocaldarius
Other publictions for EC 7.3.2.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
697446
Zolotarev
Increased sulfate uptake by E. ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Comp. Biochem. Physiol. A
149
255-266
2008
-
-
1
-
1
-
6
1
-
1
-
2
-
157
-
-
-
-
-
-
1
-
6
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
1
-
-
6
-
1
-
1
-
2
-
-
-
-
-
-
1
-
6
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
668610
Scheffel
Structure of the ATPase subuni ...
Alicyclobacillus acidocaldarius
FEBS Lett.
579
2953-2958
2005
-
-
1
1
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
441605
Sirko
Sulfate and thiosulfate transp ...
Escherichia coli
J. Bacteriol.
177
4134-4136
1995
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
441606
Jacobson
Engineered interdomain disulfi ...
Escherichia coli
J. Biol. Chem.
266
5220-5225
1991
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
441607
Laudenbach
Characterization and mutagenes ...
Marchantia polymorpha, Synechococcus sp.
J. Bacteriol.
173
2739-2750
1991
-
-
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
441608
Hryniewicz
Sulfate and thiosulfate transp ...
Escherichia coli
J. Bacteriol.
172
3358-3366
1990
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-