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Literature summary for 7.3.2.1 extracted from

  • Yadav, V.; Kumar, M.; Deep, D.K.; Kumar, H.; Sharma, R.; Tripathi, T.; Tuteja, N.; Saxena, A.K.; Johri, A.K.
    A phosphate transporter from the root endophytic fungus Piriformospora indica plays a role in phosphate transport to the host plant (2010), J. Biol. Chem., 285, 26532-26544.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Serendipita indica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
phosphate pH and temperature not specified in the publication, iPT belongs to the high affinity phosphate transporter family Serendipita indica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57700
-
calculated from cDNA Serendipita indica

Organism

Organism UniProt Comment Textmining
Serendipita indica A8N031
-
-

Source Tissue

Source Tissue Comment Organism Textmining
hypha localised to the external hyphae of Piriformospora indica colonized with maize plant root, which suggests that external hyphae are the initial site of phosphate uptake from the soil Serendipita indica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + phosphate/out
-
Serendipita indica ADP + phosphate + phosphate/in
-
?

Synonyms

Synonyms Comment Organism
phosphate transporter
-
Serendipita indica
PiPT
-
Serendipita indica

General Information

General Information Comment Organism
malfunction knockdown transformants of the gene are prepared using electroporation and RNAinterference. Knockdown transformants transport a significantly lower amount of phosphate to the host plant than wild-type. Higher amounts of phosphate are found in plants colonized with wild-type than that of non-colonized and plants colonized with knockdown PiPT Serendipita indica