Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
D228E | drastic increase in expression compared to wild-type, mutant shows no transport activity | Arabidopsis thaliana |
D382A | Km: (phosphate) 0.057 mM (in the presence of 25 mM NaCl), 0.49 mM (in the presence of various concentrations of NaCl), Vmax (adjusted to the protein expression levels): 156 nmol/mg of protein/h, results indicate that Arg-228 may participate in interactions associated with protein conformational changes required for full transport activity | Arabidopsis thaliana |
D382E | Km: (phosphate) 0.046 mM (in the presence of 25 mM NaCl), 0.73 mM (in the presence of various concentrations of NaCl), Vmax (adjusted to the protein expression levels): 96 nmol/mg of protein/h, drastic increase in expression compared to wild-type, results indicate that Arg-228 may participate in interactions associated with protein conformational changes required for full transport activity | Arabidopsis thaliana |
D382N | mutant shows no transport activity | Arabidopsis thaliana |
R120K | Km: (phosphate) 0.161 mM (in the presence of 25 mM NaCl), 1.48 mM (in the presence of various concentrations of NaCl), Vmax (adjusted to the protein expression levels): 250 nmol/mg of protein/h, results indicate that Arg-120 may be important for binding and translocation of the substrate | Arabidopsis thaliana |
R201K | Km: (phosphate) 0.214 mM (in the presence of 25 mM NaCl), 2.29 mM (in the presence of various concentrations of NaCl), Vmax (adjusted to the protein expression levels): 100 nmol/mg of protein/h, results indicate that Arg-201 may be important for binding and translocation of the substrate | Arabidopsis thaliana |
R228K | drastic increase in expression compared to wild-type, Km: (phosphate) 0.103 mM (in the presence of 25 mM NaCl), 1.99 mM (in the presence of various concentrations of NaCl), Vmax (adjusted to the protein expression levels): 274 nmol/mg of protein/h, drastic increase in expression compared to wild-type, results indicate that Arg-228 may participate in interactions associated with protein conformational changes required for full transport activity | Arabidopsis thaliana |
S124A | Km: (phosphate) 0.0448 mM (in the presence of 25 mM NaCl), 0.27 mM (in the presence of various concentrations of NaCl), Vmax (adjusted to the protein expression levels): 47 nmol/mg of protein/h, results indicate that Ser-124 may function as a transient binding site for Na+ ions in close proximity to the periplasmic side | Arabidopsis thaliana |
S124T | Km: (phosphate) 0.109 mM (in the presence of 25 mM NaCl), 0.88 mM (in the presence of various concentrations of NaCl), Vmax (adjusted to the protein expression levels): 254 nmol/mg of protein/h, results indicate that Ser-124 may function as a transient binding site for Na+ ions in close proximity to the periplasmic side | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0448 | - |
phosphate | mutant S124A, pH 6.5, 25°C, in the presence of 25 mM NaCl, Vmax (adjusted to the protein expression levels): 47 nmol/mg of protein/h | Arabidopsis thaliana | |
0.0462 | - |
phosphate | mutant D382E, pH 6.5, 25°C, in the presence of 25 mM NaCl, Vmax (adjusted to the protein expression levels): 96 nmol/mg of protein/h | Arabidopsis thaliana | |
0.0572 | - |
phosphate | mutant D382A, pH 6.5, 25°C, in the presence of 25 mM NaCl, Vmax (adjusted to the protein expression levels): 156 nmol/mg of protein/h | Arabidopsis thaliana | |
0.077 | - |
phosphate | wild-type, pH 6.5, 25°C, in the presence of 25 mM NaCl | Arabidopsis thaliana | |
0.103 | - |
phosphate | mutant R228K, pH 6.5, 25°C, in the presence of 25 mM NaCl, Vmax (adjusted to the protein expression levels): 274 nmol/mg of protein/h | Arabidopsis thaliana | |
0.109 | - |
phosphate | mutant S124T, pH 6.5, 25°C, in the presence of 25 mM NaCl, Vmax (adjusted to the protein expression levels): 254 nmol/mg of protein/h | Arabidopsis thaliana | |
0.161 | - |
phosphate | mutant R120K, pH 6.5, 25°C, in the presence of 25 mM NaCl, Vmax (adjusted to the protein expression levels): 250 nmol/mg of protein/h | Arabidopsis thaliana | |
0.214 | - |
phosphate | mutant R201K, pH 6.5, 25°C, in the presence of 25 mM NaCl, Vmax (adjusted to the protein expression levels): 100.7 nmol/mg of protein/h | Arabidopsis thaliana | |
0.27 | - |
phosphate | mutant S124A, pH 6.5, 25°C, in the presence of various concentrations of NaCl, Vmax (adjusted to the protein expression levels): 62 nmol/mg of protein/h | Arabidopsis thaliana | |
0.49 | - |
phosphate | mutant D382A, pH 6.5, 25°C, in the presence of various concentrations of NaCl, Vmax (adjusted to the protein expression levels): 119 nmol/mg of protein/h | Arabidopsis thaliana | |
0.73 | - |
phosphate | mutant D382E, pH 6.5, 25°C, in the presence of various concentrations of NaCl, Vmax (adjusted to the protein expression levels): 132 nmol/mg of protein/h | Arabidopsis thaliana | |
0.84 | - |
phosphate | wild-type, pH 6.5, 25°C, in the presence of various concentrations of NaCl | Arabidopsis thaliana | |
0.88 | - |
phosphate | mutant S124T, pH 6.5, 25°C, in the presence of various concentrations of NaCl, Vmax (adjusted to the protein expression levels): 222 nmol/mg of protein/h | Arabidopsis thaliana | |
1.48 | - |
phosphate | mutant R120K, pH 6.5, 25°C, in the presence of various concentrations of NaCl, Vmax (adjusted to the protein expression levels): 125 nmol/mg of protein/h | Arabidopsis thaliana | |
1.99 | - |
phosphate | mutant R228K, pH 6.5, 25°C, in the presence of various concentrations of NaCl, Vmax (adjusted to the protein expression levels): 221 nmol/mg of protein/h | Arabidopsis thaliana | |
2.29 | - |
phosphate | mutant R201K, pH 6.5, 25°C, in the presence of various concentrations of NaCl, Vmax (adjusted to the protein expression levels): 126 nmol/mg of protein/h | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
thylakoid membrane | - |
Arabidopsis thaliana | 42651 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Na+ | 25 mM | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
SDS-PAGE | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + phosphate/out | - |
Arabidopsis thaliana | ADP + phosphate + phosphate/in | - |
? |
Synonyms | Comment | Organism |
---|---|---|
anion transporter 1 | - |
Arabidopsis thaliana |
ANTR1 | - |
Arabidopsis thaliana |
thylakoid phosphate transporter | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Arabidopsis thaliana |