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Literature summary for 7.3.2.1 extracted from

  • Yuan, Z.C.; Zaheer, R.; Finan, T.M.
    Regulation and properties of PstSCAB, a high-affinity, high-velocity phosphate transport system of Sinorhizobium meliloti (2006), J. Bacteriol., 188, 1089-1102.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.3.2.1

Cloned(Commentary)

Cloned (Comment) Organism
gene pstSCAB, the expression is regulated by PhoB, which binds to the pstSCAB promoter at two binding sites Sinorhizobium meliloti

Protein Variants

Protein Variants Comment Organism
additional information mutation of pstC affects the PstSCAB phosphate transport system resulting in the PhoB-phenotype, overview Sinorhizobium meliloti

Inhibitors

Inhibitors Comment Organism Structure
arsenate 96% inhibition at 0.04 mM Sinorhizobium meliloti
additional information poor inhibition by ethyl phosphonate, methyl phosphonate, and aminoethyl phosphonate at 0.004-0.04 mM Sinorhizobium meliloti
potassium dihydrogen phosphate 96% inhibition at 0.04 mM Sinorhizobium meliloti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Sinorhizobium meliloti
0.0002
-
 phosphate PstSCAB, 30°C Sinorhizobium meliloti

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Sinorhizobium meliloti 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Sinorhizobium meliloti

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + phosphate/out Sinorhizobium meliloti the enzyme is involved in the phosphate uptake system in the alfalfa symbiont Sinorhizobium meliloti, PhoB has regulatory function, overview ADP + phosphate + phosphate/in
-
 ?

Organism

Organism UniProt Comment Textmining
Sinorhizobium meliloti
-
 alfalfa symbiont in root nodules, strain 1021, gene pstSCAB
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + phosphate/out the enzyme is involved in the phosphate uptake system in the alfalfa symbiont Sinorhizobium meliloti, PhoB has regulatory function, overview Sinorhizobium meliloti ADP + phosphate + phosphate/in
-
 ?
ATP + H2O + phosphate/out PstSCAB is specific for phosphate Sinorhizobium meliloti ADP + phosphate + phosphate/in
-
 ?

Synonyms

Synonyms Comment Organism
PstSCAB
-
 Sinorhizobium meliloti

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Sinorhizobium meliloti