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Literature summary for 7.2.4.3 extracted from

  • Hilpert, W.; Dimroth, P.
    Sodium pump methylmalonyl-CoA decarboxylase from Veillonella alcalescens (1986), Methods Enzymol., 125, 540-546.
No PubMed abstract available
Search for more literature for 7.2.4.3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
 (S)-methylmalonyl-CoA at 20 mM NaCl Veillonella parvula
0.035
-
 malonyl-CoA at 20 mM NaCl Veillonella parvula

Localization

Localization Comment Organism GeneOntology No. Textmining
cell membrane firmly bound Veillonella parvula
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Na+ activates Veillonella parvula

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14000
-
 x * 60000, alpha, + x * 33000, beta, + x * 18500, gamma, + x * 14000, delta, SDS-PAGE Veillonella parvula
18500
-
 x * 60000, alpha, + x * 33000, beta, + x * 18500, gamma, + x * 14000, delta, SDS-PAGE Veillonella parvula
33000
-
 x * 60000, alpha, + x * 33000, beta, + x * 18500, gamma, + x * 14000, delta, SDS-PAGE Veillonella parvula
60000
-
 x * 60000, alpha, + x * 33000, beta, + x * 18500, gamma, + x * 14000, delta, SDS-PAGE Veillonella parvula

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-methylmalonyl-CoA + Na+[side 1] + H+[side 2] Veillonella parvula the enzyme is one of the sodium pumps, which are driven by decarboxylation energy propanoyl-CoA + CO2 + Na+[side 2]
-
 ?
(S)-methylmalonyl-CoA + Na+[side 1] + H+[side 2] Veillonella parvula reconstitution of the sodium pump propanoyl-CoA + CO2 + Na+[side 2]
-
 ?

Organism

Organism UniProt Comment Textmining
Veillonella parvula
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Veillonella parvula

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Veillonella parvula

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-methylmalonyl-CoA + Na+[side 1] + H+[side 2]
-
 Veillonella parvula propanoyl-CoA + CO2 + Na+[side 2]
-
 ?
(S)-methylmalonyl-CoA + Na+[side 1] + H+[side 2] the enzyme is one of the sodium pumps, which are driven by decarboxylation energy Veillonella parvula propanoyl-CoA + CO2 + Na+[side 2]
-
 ?
(S)-methylmalonyl-CoA + Na+[side 1] + H+[side 2] reconstitution of the sodium pump Veillonella parvula propanoyl-CoA + CO2 + Na+[side 2]
-
 ?
Malonyl-CoA
-
 Veillonella parvula Acetyl-CoA + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, alpha, + x * 33000, beta, + x * 18500, gamma, + x * 14000, delta, SDS-PAGE Veillonella parvula

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4 7
-
 Veillonella parvula

Cofactor

Cofactor Comment Organism Structure
biotin is contained in the gamma subunit Veillonella parvula