BRENDA - Enzyme Database show
show all sequences of 7.2.4.2

Membrane topology of the beta-subunit of the oxaloacetate decarboxylase Na+ pump from Klebsiella pneumoniae

Jockel, P.; Di Berardino, M.; Dimroth, P.; Biochemistry 38, 13461-13472 (1999)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli CC118
Klebsiella pneumoniae
Engineering
Amino acid exchange
Commentary
Organism
C87A
slightly increased activity
Klebsiella pneumoniae
C87A/A194C
no effect on activity
Klebsiella pneumoniae
C87A/I198C
no effect on activity
Klebsiella pneumoniae
C87A/L178C
about 50% of activity
Klebsiella pneumoniae
C87A/L7C
no activity
Klebsiella pneumoniae
C87A/P191C
no activity
Klebsiella pneumoniae
C87A/P205C
no effect on activity; only traces of activity
Klebsiella pneumoniae
C87A/S187C
no effect on activity
Klebsiella pneumoniae
C87A/V129C
strongly increased activity
Klebsiella pneumoniae
C87A/Y182C
slightly increased activity
Klebsiella pneumoniae
additional information
fusion protein from different domains of the enzyme subunits
Klebsiella pneumoniae
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
beta and gamma subunits are integral membrane proteins, alpha subunit is attached to the gamma subunit
Klebsiella pneumoniae
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Zn2+
binds to the gamma subunit
Klebsiella pneumoniae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
Oxaloacetate
Klebsiella pneumoniae
one Na+ is transported into the periplasm and one H+ is transported into the cytoplasm during the reaction
Pyruvate + CO2
-
Klebsiella pneumoniae
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Klebsiella pneumoniae
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Oxaloacetate
reaction is catalyzed at low rate by the alpha subunit alone, much higher activity with the complete enzyme
649873
Klebsiella pneumoniae
Pyruvate + CO2
-
649873
Klebsiella pneumoniae
?
Oxaloacetate
one Na+ is transported into the periplasm and one H+ is transported into the cytoplasm during the reaction
649873
Klebsiella pneumoniae
Pyruvate + CO2
-
649873
Klebsiella pneumoniae
?
Subunits
Subunits
Commentary
Organism
trimer
alpha, beta, gamma
Klebsiella pneumoniae
Cofactor
Cofactor
Commentary
Organism
Structure
biotin
binds to the C-terminal domain of the alpha-subunit
Klebsiella pneumoniae
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli CC118
Klebsiella pneumoniae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
biotin
binds to the C-terminal domain of the alpha-subunit
Klebsiella pneumoniae
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C87A
slightly increased activity
Klebsiella pneumoniae
C87A/A194C
no effect on activity
Klebsiella pneumoniae
C87A/I198C
no effect on activity
Klebsiella pneumoniae
C87A/L178C
about 50% of activity
Klebsiella pneumoniae
C87A/L7C
no activity
Klebsiella pneumoniae
C87A/P191C
no activity
Klebsiella pneumoniae
C87A/P205C
no effect on activity; only traces of activity
Klebsiella pneumoniae
C87A/S187C
no effect on activity
Klebsiella pneumoniae
C87A/V129C
strongly increased activity
Klebsiella pneumoniae
C87A/Y182C
slightly increased activity
Klebsiella pneumoniae
additional information
fusion protein from different domains of the enzyme subunits
Klebsiella pneumoniae
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
beta and gamma subunits are integral membrane proteins, alpha subunit is attached to the gamma subunit
Klebsiella pneumoniae
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Zn2+
binds to the gamma subunit
Klebsiella pneumoniae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
Oxaloacetate
Klebsiella pneumoniae
one Na+ is transported into the periplasm and one H+ is transported into the cytoplasm during the reaction
Pyruvate + CO2
-
Klebsiella pneumoniae
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Oxaloacetate
reaction is catalyzed at low rate by the alpha subunit alone, much higher activity with the complete enzyme
649873
Klebsiella pneumoniae
Pyruvate + CO2
-
649873
Klebsiella pneumoniae
?
Oxaloacetate
one Na+ is transported into the periplasm and one H+ is transported into the cytoplasm during the reaction
649873
Klebsiella pneumoniae
Pyruvate + CO2
-
649873
Klebsiella pneumoniae
?
Subunits (protein specific)
Subunits
Commentary
Organism
trimer
alpha, beta, gamma
Klebsiella pneumoniae
Other publictions for EC 7.2.4.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749221
Inoue
Highly active and stable oxal ...
Vibrio cholerae
Protein Expr. Purif.
115
34-38
2015
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
1
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
650112
Schmid
Subunit gamma of the oxaloacet ...
Klebsiella pneumoniae
Biochemistry
41
1285-1292
2002
-
-
1
-
8
-
-
-
1
1
-
1
-
1
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
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1
1
-
8
-
-
-
-
-
1
1
-
1
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
649957
Jockel
A molecular coupling mechanism ...
Klebsiella pneumoniae
Biochemistry
39
2307-2315
2000
-
-
1
-
18
-
-
-
1
2
-
1
-
1
-
-
1
-
-
-
-
-
2
1
-
-
-
-
2
-
-
1
-
-
-
-
-
1
1
-
18
-
-
-
-
-
1
2
-
1
-
-
-
1
-
-
-
-
2
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
649974
Jockel
Essential role of tyrosine 229 ...
Klebsiella pneumoniae
Biochemistry
39
4320-4326
2000
-
-
1
-
20
-
-
-
1
2
-
1
-
1
-
-
1
-
-
-
2
-
2
1
-
-
-
-
2
-
-
1
-
-
-
-
-
1
1
-
20
-
-
-
-
-
1
2
-
1
-
-
-
1
-
-
2
-
2
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
649873
Jockel
Membrane topology of the beta- ...
Klebsiella pneumoniae
Biochemistry
38
13461-13472
1999
-
-
1
-
11
-
-
-
1
1
-
1
-
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
11
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4277
Di Bernardino
Cellular localisation by immun ...
Klebsiella pneumoniae
FEMS Microbiol. Lett.
136
31-37
1996
-
-
1
-
-
-
-
-
2
1
-
1
-
1
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
1
-
1
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4274
Di Bernardino
Synthesis of the oxaloacetate ...
Klebsiella pneumoniae
Eur. J. Biochem.
231
790-801
1995
-
-
1
-
-
-
-
-
-
2
-
1
-
1
-
-
1
-
-
-
1
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
1
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4282
Dimroth
On the mechanism of sodium ion ...
Klebsiella pneumoniae
Biochemistry
32
1734-1739
1993
-
-
-
-
-
-
-
-
-
1
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1
-
1
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3
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1
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1
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3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4280
Dimroth
The sodium ion pumping oxaloac ...
Klebsiella pneumoniae
FEBS Lett.
300
67-70
1992
-
-
-
-
-
-
4
-
-
2
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
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-
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-
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-
-
4
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2
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4278
Wifling
Isolation and characterization ...
Salmonella enterica subsp. enterica serovar Typhimurium
Arch. Microbiol.
152
584-588
1989
-
-
-
-
-
-
1
-
-
1
3
2
-
1
-
-
1
-
-
-
1
-
4
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
1
3
2
-
-
-
1
-
-
1
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4258
Schwarz
The sodium ion translocating o ...
Klebsiella pneumoniae
J. Biol. Chem.
263
9640-9645
1988
-
-
1
-
-
-
-
-
-
1
3
1
-
1
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
3
1
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4259
Dimroth
Dissociation of the sodium-ion ...
Klebsiella pneumoniae
Eur. J. Biochem.
175
175-180
1988
-
-
-
-
-
-
-
-
1
1
-
1
-
1
-
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-
1
-
-
-
3
-
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1
1
-
1
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1
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4281
Daekena
-
Oxaloacetate decarboxylase fro ...
Klebsiella pneumoniae
FEMS Microbiol. Lett.
55
35-40
1988
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
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1
-
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3
1
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1
-
1
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1
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3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4260
Dimroth
Preparation, characterization, ...
Klebsiella aerogenes
Methods Enzymol.
125
530-540
1986
-
-
-
-
-
-
5
1
1
1
3
1
-
1
-
-
1
1
-
-
2
-
4
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
5
-
1
1
1
3
1
-
-
-
1
-
-
2
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
4261
Dimroth
Kinetic analysis of the reacti ...
Klebsiella aerogenes
Eur. J. Biochem.
156
157-162
1986
-
-
-
-
-
-
4
-
1
2
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
4
4
-
1
2
-
1
-
-
-
-
-
-
-
-
3
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
4279
Dimroth
-
Carboxylation of pyruvate and ...
Klebsiella aerogenes
Biochemistry
23
5360-5366
1984
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
4
-
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1
-
1
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-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4262
Dimroth
Subunit composition of oxaloac ...
Klebsiella aerogenes
Eur. J. Biochem.
137
107-112
1983
-
-
-
-
-
-
-
-
1
1
3
1
-
1
-
-
1
-
-
-
1
-
3
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
1
3
1
-
-
-
1
-
-
1
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4264
Dimroth
Purification of the sodium tra ...
Klebsiella aerogenes
FEBS Lett.
141
59-62
1982
-
-
-
-
-
-
-
-
1
1
-
1
-
1
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
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-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4265
Dimroth
The generation of an electroch ...
Klebsiella aerogenes
Eur. J. Biochem.
121
443-449
1982
-
-
-
-
-
-
1
-
1
1
-
2
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
1
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4266
Dimroth
The role of biotin and sodium ...
Klebsiella aerogenes
Eur. J. Biochem.
121
435-441
1982
-
-
-
-
-
-
-
1
1
1
-
1
-
1
-
-
-
1
-
-
-
-
3
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
4267
Dimroth
Characterization of a membrane ...
Klebsiella aerogenes
Eur. J. Biochem.
115
353-358
1981
-
-
-
-
-
1
6
1
1
1
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
1
-
-
1
-
6
1
1
1
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
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