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Literature summary for 7.2.4.2 extracted from

  • Jockel, P.; Di Berardino, M.; Dimroth, P.
    Membrane topology of the beta-subunit of the oxaloacetate decarboxylase Na+ pump from Klebsiella pneumoniae (1999), Biochemistry, 38, 13461-13472.
    View publication on PubMed
Search for more literature for 7.2.4.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli CC118 Klebsiella pneumoniae

Protein Variants

Protein Variants Comment Organism
C87A slightly increased activity Klebsiella pneumoniae
C87A/A194C no effect on activity Klebsiella pneumoniae
C87A/I198C no effect on activity Klebsiella pneumoniae
C87A/L178C about 50% of activity Klebsiella pneumoniae
C87A/L7C no activity Klebsiella pneumoniae
C87A/P191C no activity Klebsiella pneumoniae
C87A/P205C no effect on activity Klebsiella pneumoniae
C87A/P205C only traces of activity Klebsiella pneumoniae
C87A/S187C no effect on activity Klebsiella pneumoniae
C87A/V129C strongly increased activity Klebsiella pneumoniae
C87A/Y182C slightly increased activity Klebsiella pneumoniae
additional information fusion protein from different domains of the enzyme subunits Klebsiella pneumoniae

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane beta and gamma subunits are integral membrane proteins, alpha subunit is attached to the gamma subunit Klebsiella pneumoniae 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ binds to the gamma subunit Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Oxaloacetate Klebsiella pneumoniae one Na+ is transported into the periplasm and one H+ is transported into the cytoplasm during the reaction Pyruvate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Oxaloacetate reaction is catalyzed at low rate by the alpha subunit alone, much higher activity with the complete enzyme Klebsiella pneumoniae Pyruvate + CO2
-
 ?
Oxaloacetate one Na+ is transported into the periplasm and one H+ is transported into the cytoplasm during the reaction Klebsiella pneumoniae Pyruvate + CO2
-
 ?

Subunits

Subunits Comment Organism
trimer alpha, beta, gamma Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
biotin binds to the C-terminal domain of the alpha-subunit Klebsiella pneumoniae