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Literature summary for 7.2.2.9 extracted from

  • Mana-Capelli, S.; Mandal, A.K.; Argüello, J.M.
    Archaeoglobus fulgidus CopB is a thermophilic Cu2+-ATPase: functional role of its histidine-rich-N-terminal metal binding domain (2003), J. Biol. Chem., 278, 40534-40541.
    View publication on PubMed
Search for more literature for 7.2.2.9

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Archaeoglobus fulgidus

Protein Variants

Protein Variants Comment Organism
delM1-M54 a truncated CopB lacking the first 54 amino acids is constructed to characterize the N-terminal metal binding domain. This enzyme shows reduced ATPase activity (50% of wild type) but no changes in metal selectivity, ATP dependence, or phosphorylation levels Archaeoglobus fulgidus

Metals/Ions

Metals/Ions Comment Organism Structure
Ag+ partially activated by Ag+, 55%, of the activation compared to Cu2+ Archaeoglobus fulgidus
Cu+ partially activated by Cu+, 22% of the activation compared to Cu2+ Archaeoglobus fulgidus
Cu2+ activated by Cu2+ with high apparent affinity (K1/2 = 0.00028 mM) Archaeoglobus fulgidus
additional information CopB and its homologs are distinguished by a metal binding sequence Cys-Pro-His in their sixth transmembrane segment and a His-rich N-terminal metal binding domain Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + Cu2+in Archaeoglobus fulgidus CopB drives the efflux of Cu2+ from the cell ADP + phosphate + Cu2+out
-
 ?

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus O30085
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Cu2+in CopB drives the efflux of Cu2+ from the cell Archaeoglobus fulgidus ADP + phosphate + Cu2+out
-
 ?
ATP + H2O + Cu2+in the presence of the His residue in the putative transmembrane metal binding site of CopB determines a selectivity for this enzyme that is different for that observed in Cu+/Ag+-ATPases carrying a Cys-Pro-Cys sequence Archaeoglobus fulgidus ADP + phosphate + Cu2+out
-
 ?

Synonyms

Synonyms Comment Organism
CopB the enzyme is a member of the P1B-3-subgroup with a Cys-Pro-His sequence in H6 and 17 His residues within the first 51 amino acids Archaeoglobus fulgidus
Cu2+-ATPase
-
 Archaeoglobus fulgidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 assay at Archaeoglobus fulgidus

General Information

General Information Comment Organism
physiological function CopB drives the efflux of Cu2+ from the cell Archaeoglobus fulgidus