Literature summary for 7.2.2.9 extracted from

Veldhuis, N.A.; Valova, V.A.; Gaeth, A.P.; Palstra, N.; Hannan, K.M.; Michell, B.J.; Kelly, L.E.; Jennings, I.; Kemp, B.E.; Pearson, R.B.; Robinson, P.J.; Camakaris, J.
Phosphorylation regulates copper-responsive trafficking of the Menkes copper transporting P-type ATPase (2009), Int. J. Biochem. Cell Biol., 41, 2403-2412.

Search for more literature for 7.2.2.9

Cloned(Commentary)

Cloned (Comment)	Organism
expression of mutant enzymes in MDCK cells and in CHO cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
S1432A	site-directed mutagenesis of a constitutively phosphorylated site, mutation leads to mislocation of the enzyme in presence of Cu2+	Homo sapiens
S1469A	site-directed mutagenesis of a copper-responsive phosphorylation site, mutation leads to mislocation of the enzyme in presence of Cu2+	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
Golgi membrane	-	Cricetulus griseus	139	-
Golgi membrane	-	Homo sapiens	139	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cu2+	copper stimulates both trafficking and phosphorylation of ATP7A	Cricetulus griseus
Cu2+	copper stimulates both trafficking and phosphorylation of ATP7A	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + Cu2+/in	Cricetulus griseus	copper stimulates both trafficking and phosphorylation of ATP7A	ADP + phosphate + Cu2+/out	-	?
ATP + H2O + Cu2+/in	Homo sapiens	copper stimulates both trafficking and phosphorylation of ATP7A	ADP + phosphate + Cu2+/out	-	?

Organism

Organism	UniProt	Comment	Textmining
Cricetulus griseus	-	gene ATP7A	-
Homo sapiens	-	gene ATP7A	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	copper stimulates both trafficking and phosphorylation of ATP7A, identification of eight in vivo phosphorylation sites in the hamster ATP7A, all clustered within the N- and C-terminal cytosolic domains, C- and N-terminal phosphorylation sites, overview	Cricetulus griseus
phosphoprotein	copper stimulates both trafficking and phosphorylation of ATP7A, identification of twenty in vivo phosphorylation sites in the human ATP7A, all clustered within the N- and C-terminal cytosolic domains, eight sites are copper-responsive and hence candidates for regulating copper-responsive trafficking or catalytic activity. A constitutively phosphorylated site is Ser1432, a copper-responsive phosphorylation site Ser1469. Other C-terminal phosphorylation sites are serine residues 1463, 1466, 1469, 1473, 1476 and 1480, C- and N-termninal phosphorylation sites, overview	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
CHO-CUR3 cell	-	Cricetulus griseus	-
CHO-K1 cell	-	Cricetulus griseus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + Cu2+/in	-	Cricetulus griseus	ADP + phosphate + Cu2+/out	-	?
ATP + H2O + Cu2+/in	-	Homo sapiens	ADP + phosphate + Cu2+/out	-	?
ATP + H2O + Cu2+/in	copper stimulates both trafficking and phosphorylation of ATP7A	Cricetulus griseus	ADP + phosphate + Cu2+/out	-	?
ATP + H2O + Cu2+/in	copper stimulates both trafficking and phosphorylation of ATP7A	Homo sapiens	ADP + phosphate + Cu2+/out	-	?

Subunits

Subunits	Comment	Organism
More	peptide mapping and identification of putative Cu-responsive peptides, overview	Cricetulus griseus
More	peptide mapping and identification of putative Cu-responsive peptides, overview	Homo sapiens

Synonyms

Synonyms	Comment	Organism
ATP7A	-	Cricetulus griseus
ATP7A	-	Homo sapiens
copper transporting P-type ATPase	-	Cricetulus griseus
Cu-translocating P-type ATPase	-	Cricetulus griseus
Cu-translocating P-type ATPase	-	Homo sapiens
Menkes copper transporting P-type ATPase	-	Homo sapiens
MNK	-	Homo sapiens

General Information

General Information	Comment	Organism
additional information	specific serine residues in ATP7A regulates its sub-cellular localization and hence function and will facilitate identification of the kinases and signaling pathways involved in regulating this pivotal copper transporter	Cricetulus griseus
additional information	specific serine residues in ATP7A regulates its sub-cellular localization and hence function and will facilitate identification of the kinases and signaling pathways involved in regulating this pivotal copper transporter. C-terminal MNK phosphorylation is essential for copper-responsive trafficking	Homo sapiens
physiological function	Menkes copper-translocating P-type ATPase, ATP7A, is a critical copper transport protein functioning in systemic copper absorption and supply of copper to cuproenzymes in the secretory pathway. Mutations in ATP7A can lead to the usually lethal Menkes disease. ATP7A function is regulated by copper-responsive trafficking between the trans-Golgi Network and the plasma membrane	Homo sapiens

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Release 2023.1 (January 2023)