Literature summary for 7.2.2.8 extracted from

Bredeston, L.M.; Gonzalez Flecha, F.L.
The promiscuous phosphomonoestearase activity of Archaeoglobus fulgidus CopA, a thermophilic Cu+ transport ATPase (2016), Biochim. Biophys. Acta, 1858, 1471-1478 .

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Activating Compound

Activating Compound	Comment	Organism	Structure
Phospholipid	addition of phospholipids leads to a significant increase in CopA phosphomonoesterase turnover	Archaeoglobus fulgidus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.11	-	ATP	pH 6.1, 75°C	Archaeoglobus fulgidus
9	-	4-nitrophenyl phosphate	pH 6.1, 75°C	Archaeoglobus fulgidus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	essential activator for the hydrolysis of 4-nitrophenylphosphate	Archaeoglobus fulgidus
additional information	no effect on the reaction with 4-nitrophenylphosphate: Mn2+, Co2+, Sr2+, Ba2+	Archaeoglobus fulgidus

Organism

Organism	UniProt	Comment	Textmining
Archaeoglobus fulgidus	O29777	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl phosphate + H2O + Cu+[side 1]	-	Archaeoglobus fulgidus	4-nitrophenol + phosphate + Cu+[side 2]	-	?
ATP + H2O + Cu+[side 1]	catalytic proficiency of CopA is slightly higher toward its natural substrate ATP than toward 4-nitrophenyl phosphate	Archaeoglobus fulgidus	ADP + phosphate + Cu+[side 2]	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
70	80	hydrolysis of 4-nitrophenyl phosphate	Archaeoglobus fulgidus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.26	-	ATP	pH 6.1, 75°C	Archaeoglobus fulgidus
5.9	-	4-nitrophenyl phosphate	pH 6.1, 75°C	Archaeoglobus fulgidus

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Release 2023.1 (January 2023)