Literature summary for 7.2.2.8 extracted from

Jain, S.; Farias, G.G.; Bonifacino, J.S.
Polarized sorting of the copper transporter ATP7B in neurons mediated by recognition of a dileucine signal by AP-1 (2015), Mol. Biol. Cell, 26, 218-228.

Search for more literature for 7.2.2.8

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of His-tagged MBP-fusion enzyme in Escherichia coli, overexpression of GFP-tagged ATP7B in Rattus norvegicus hippocampal neurons, co-expression with myc-tagged wild-type or V98S-mutant sigma1A, with HA-tagged wild-type or V98S-mutant sigma1B, and with HA-tagged wild-type or V98S-mutant sigma1C	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
endosome	high Cu2+ concentration redistributes ATP7B to late endosomes or lysosomes that move along the axon in live hippocampal neurons	Homo sapiens	5768	-
lysosome	high Cu2+ concentration redistributes ATP7B to late endosomes or lysosomes that move along the axon in live hippocampal neurons	Homo sapiens	5764	-
plasma membrane	under basal Cu2+ conditions, ATP7B is localized to the trans-Golgi network and the plasma membrane of the soma and dendrites but not the axon	Homo sapiens	5886	-
trans-Golgi network	Cu2+-regulated, dileucine-dependent localization of ATP7B to the neuronal trans-Golgi network. Under basal Cu2+ conditions, ATP7B is localized to the trans-Golgi network and the plasma membrane of the soma and dendrites but not the axon. Addition of high Cu2+ concentrations cause loss of trans-Golgi network localization and somatodendritic polarity of ATP7B. ATP7B co-localizes with sigma1B, as well as with the endogenous gamma subunit at the trans-Golgi network	Homo sapiens	5802	-
vesicle	ATP7B incorporation into AP-1-containing clathrin-coated vesicles. Addition of high Cu2+ concentrations reduce ATP7B incorporation into AP-1-containing clathrin-coated vesicle	Homo sapiens	31982	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P35670	ATP7B	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged MBP-fusion enzyme from Escherichia coli by nickel affinity chromatography and gel filtration	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	select areas of the brain, including the developing central nervous system	Homo sapiens	-
central nervous system	-	Homo sapiens	-
cerebellum	-	Homo sapiens	-
hippocampus	high Cu2+ concentration redistributes ATP7B to late endosomes or lysosomes that move along the axon in live hippocampal neurons	Homo sapiens	-
liver	ATP7B is mainly expressed in the liver	Homo sapiens	-
neuron	enzyme ATP7B is localized to the trans-Golgi network and the plasma membrane of the soma and dendrites but not the axon. Addition of high Cu2+ concentrations cause loss of somatodendritic polarity of ATP7B. High Cu2+ concentration redistributes ATP7B to late endosomes or lysosomes that move along the axon in live hippocampal neurons	Homo sapiens	-

Synonyms

Synonyms	Comment	Organism
ATP7B	-	Homo sapiens
copper transporter	-	Homo sapiens

General Information

General Information	Comment	Organism
malfunction	addition of high Cu2+ concentrations reduce ATP7B incorporation into AP-1-containing clathrin-coated vesicles and cause loss of trans-Golgi network localization and somatodendritic polarity of ATP7B	Homo sapiens
physiological function	the enzyme binds to the gamma-subunit of AP-1, an effector of polarized sorting in neurons, via dileucine-dependent binding of the C-terminal tail of ATP7B. Interaction with AP-1 is critical for somatodendritic polarity of ATP7B. Altered polarity of ATP7B in polarized cell types might contribute to abnormal copper metabolism in the MEDNIK syndrome, a neurocutaneous disorder caused by mutations in the sigma1A subunit isoform of AP-1	Homo sapiens

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Release 2023.1 (January 2023)