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Literature summary for 7.2.2.6 extracted from

  • Siebers, A.; Altendorf, K.
    The K+-translocating Kdp-ATPase from Escherichia coli. Purification, enzymatic properties and production of complex- and subunit-specific antisera (1988), Eur. J. Biochem., 178, 131-140.
    View publication on PubMed
Search for more literature for 7.2.2.6

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Escherichia coli
Fluorescein isothiocyanate
-
 Escherichia coli
N,N'-dicyclohexylcarbodiimide
-
 Escherichia coli
N-ethylmaleimide
-
 Escherichia coli
ortho-vanadate
-
 Escherichia coli
Tl+
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 K+
-
 Escherichia coli
0.08
-
 ATP
-
 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+
-
 Escherichia coli
K+
-
 Escherichia coli
Mn2+
-
 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
 1 * 79000 + 1 * 74000 + 1 * 22000, SDS-PAGE Escherichia coli
74000
-
 1 * 79000 + 1 * 74000 + 1 * 22000, SDS-PAGE Escherichia coli
79000
-
 1 * 79000 + 1 * 74000 + 1 * 22000, SDS-PAGE Escherichia coli
175000
-
 SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + K+/out Escherichia coli
-
 ADP + phosphate + K+/in
-
 ?
ATP + H2O + K+/out Escherichia coli TKA 1000
-
 ADP + phosphate + K+/in
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli TKA 1000
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.567
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + K+/out
-
 Escherichia coli ADP + phosphate + K+/in
-
 ?
ATP + H2O + K+/out
-
 Escherichia coli TKA 1000 ADP + phosphate + K+/in
-
 ?
ATP + H2O + K+[side 1] ATP in form of MgATP2- Escherichia coli ADP + phosphate + K+[side 2]
-
 ?
ATP + H2O + K+[side 1] ATP in form of MgATP2- Escherichia coli TKA 1000 ADP + phosphate + K+[side 2]
-
 ?

Subunits

Subunits Comment Organism
trimer 1 * 79000 + 1 * 74000 + 1 * 22000, SDS-PAGE Escherichia coli