Application | Comment | Organism |
---|---|---|
biotechnology | expression of enzyme in Saccharomyces cerevisiae strikingly decreases Cd2+ tolerance of yeast cells. Yeast expressing the non-functional mutant D398A can grow on selective medium containing up to 0.1 mM Cd2+, while those expressing the intact enzyme cannot grow in presence of 0.001 mM Cd2+. Enzyme is localized in the endoplasmic reticulum, so hypersensitivity to Cd2+ is due to Cd2+ accumulation in the reticulum lumen. Zn2+ does not protect cells against Cd2+ poisoning | Listeria monocytogenes |
Protein Variants | Comment | Organism |
---|---|---|
D398A | no enzymic activity | Listeria monocytogenes |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | recombinant enzyme in Saccharomyces cerevisiae | Listeria monocytogenes | 5783 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Listeria monocytogenes | - |
expression in Saccharomyces cerevisiae | - |