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Literature summary for 7.2.2.20 extracted from

  • Handali, M.; Neupane, D.; Roychowdhury, H.; Yukl, E.
    Transcriptional regulation, metal binding properties and structure of Pden1597, an unusual zinc transport protein from Paracoccus denitrificans (2015), J. Biol. Chem., 290, 11878-11889 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.2.2.20

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Paracoccus denitrificans

Organism

Organism UniProt Comment Textmining
Paracoccus denitrificans
-
-
-
Paracoccus denitrificans Pd1222
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Sephacryl S-100 gel filtration Paracoccus denitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Mn2+-[manganese-binding protein][side1] the enzyme binds Zn2+ with an affinity about 100fold greater than that for Mn2+ Paracoccus denitrificans ADP + phosphate + Mn2+[side2] + [manganese-binding protein][side2]
-
 ?
ATP + H2O + Mn2+-[manganese-binding protein][side1] the enzyme binds Zn2+ with an affinity about 100fold greater than that for Mn2+ Paracoccus denitrificans Pd1222 ADP + phosphate + Mn2+[side2] + [manganese-binding protein][side2]
-
 ?
ATP + H2O + Zn2+-[zinc-binding protein][side1] the enzyme binds Zn2+ with an affinity about 100fold greater than that for Mn2+ Paracoccus denitrificans ADP + phosphate + Zn2+[side2] + [zinc-binding protein][side2]
-
 ?
ATP + H2O + Zn2+-[zinc-binding protein][side1] the enzyme binds Zn2+ with an affinity about 100fold greater than that for Mn2+ Paracoccus denitrificans Pd1222 ADP + phosphate + Zn2+[side2] + [zinc-binding protein][side2]
-
 ?

Synonyms

Synonyms Comment Organism
Pden1597
-
 Paracoccus denitrificans
Zn2+-specific transporter
-
 Paracoccus denitrificans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 the Zn2+-bound protein is remarkably stable, maintaining its circular dichroism spectrum up to 90°C Paracoccus denitrificans

Expression

Organism Comment Expression
Paracoccus denitrificans enzyme expression is increased under conditions of Zn2+ starvation up