Protein Variants | Comment | Organism |
---|---|---|
K669M | enzyme Ycf1p containing a mutation of the conserved Lys669 to Met in the Walker A box of the first nucleotide-binding domain (Ycf1pK669M) is unable to complement the fusion defect of DELTAycf1 vacuoles | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
vacuole | - |
Saccharomyces cerevisiae | 5773 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharomyces cerevisiae | enzyme Ycf1p regulates phosphatidylinositol 3-phosphate accumulation at vertex microdomains | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P39109 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme Ycf1p regulates phosphatidylinositol 3-phosphate accumulation at vertex microdomains | Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Ycf1p | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the ATP-binding cassette class C (ABCC) transporter family | Saccharomyces cerevisiae |
malfunction | deletion of YCF1 attenuates in vitro vacuole fusion by up to 40% relative to wild-type vacuoles, plasmid-expressed wild-type Ycf1p rescues the deletion phenotype. Ycf1p containing a mutation of the conserved Lys669 to Met in the Walker A box of the first nucleotide-binding domain (Ycf1pK669M) is unable to complement the fusion defect of DELTAycf1 vacuoles. Also, deleting YCF1 causes a striking decrease in vacuolar levels of the soluble SNARE Vam7p, whereas total cellular levels are not altered. The attenuated fusion of DELTAycf1 vacuoles is rescued by the addition of recombinant Vam7p to in vitro experiments, exogenous Vam7p restores Ca2+ efflux in DELTAycf1 vacuoles. Phenotypes, overview | Saccharomyces cerevisiae |
physiological function | the enzyme Ycf1p is a Cd2+ transporter, it regulates the transport of cadmium, mercury, and other toxins into the vacuole lumen to detoxify the cytoplasm. The ATPase activity of Ycf1p is required for its function in regulating fusion. The enzyme physically interacts with a wide array of proteins, including factors that regulate vacuole homeostasis. Role of Ycf1p and other ABCC transporters in the regulation of vacuole homotypic fusion, overview. Ycf1p contributes in the recruitment of Vam7p to the vacuole for efficient membrane fusion | Saccharomyces cerevisiae |