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Literature summary for 7.2.2.2 extracted from

  • Li, Z.S.; Szczypka, M.; Lu, Y.P.; Thiele ,D.J.; Rea, P.A.
    The yeast cadmium factor protein (YCF1) is a vacuolar glutathione S-conjugate pump (1996), J. Biol. Chem., 217, 6509-6517.
No PubMed abstract available
Search for more literature for 7.2.2.2

Inhibitors

Inhibitors Comment Organism Structure
vanadate IC50: 0.18 mM Saccharomyces cerevisiae
verapamil IC50: 0.2 mM Saccharomyces cerevisiae
vinblastine IC50: 0.08 mM Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000758
-
 leukotriene C4 [side 1] for Sf/YCF1-His vesicles Saccharomyces cerevisiae
0.00082
-
 leukotriene C4 [side 1]
-
 Saccharomyces cerevisiae
0.055
-
 ATP
-
 Saccharomyces cerevisiae
0.094
-
 ATP for Sf/YCF1-His vesicles Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
tonoplast
-
 Saccharomyces cerevisiae
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 1515 amino acids Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Cd/cytoplasm + ATP + H2O Saccharomyces cerevisiae
-
 Cd/vacuole + ADP + phosphate
-
 ?
S-(2,4-dinitrophenyl)glutathione/cytoplasm + ATP + H2O Saccharomyces cerevisiae
-
 S-(2,4-dinitrophenyl)glutathione/vacuole + ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Storage Stability

Storage Stability Organism
-85°C, vacuolar membrane preparations Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + leukotriene C4 [side 1]
-
 Saccharomyces cerevisiae ADP + phosphate + leukotriene C4 [side 2]
-
 ?
Cd/cytoplasm + ATP + H2O
-
 Saccharomyces cerevisiae Cd/vacuole + ADP + phosphate
-
 ?
glutathione S-conjugated leukotriene C4/cytoplasm + ATP + H2O
-
 Saccharomyces cerevisiae glutathione S-conjugated leukotriene C4/vacuole + ADP + phosphate
-
 ?
S-(2,4-dinitrophenyl)glutathione/cytoplasm + ATP + H2O
-
 Saccharomyces cerevisiae S-(2,4-dinitrophenyl)glutathione/vacuole + ADP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
Ycf1p
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Saccharomyces cerevisiae

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.08
-
 IC50: 0.08 mM Saccharomyces cerevisiae vinblastine
0.18
-
 IC50: 0.18 mM Saccharomyces cerevisiae vanadate
0.2
-
 IC50: 0.2 mM Saccharomyces cerevisiae verapamil