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Literature summary for 7.2.2.19 extracted from

  • De Pont, J.J.; Swarts, H.G.; Karawajczyk, A.; Schaftenaar, G.; Willems, P.H.; Koenderink, J.B.
    The non-gastric H,K-ATPase as a tool to study the ouabain-binding site in Na,K-ATPase (2009), Pflugers Arch., 457, 623-634.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
H+
-
Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
expression of alpha-subunit of wild-type and mutant enzymes in Spodopera frugiperda Sf9 cell membranes using the baculovirus transfection system Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
D312E/S319G/A778P/I795L/F802C a ouabain-sensitive mutant of the non-gastric H,K-ATPase, with the mutant enzyme, strophanthidin and dihydroouabain have a higher and digoxin has a lower affinity than ouabain Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
digoxigenin inhibition of mutant D312E/S319G/A778P/I795L/F802C, not of the wild-type enzyme Rattus norvegicus
digoxin inhibition of mutant D312E/S319G/A778P/I795L/F802C, not of the wild-type enzyme Rattus norvegicus
dihydro-ouabain inhibition of mutant D312E/S319G/A778P/I795L/F802C, not of the wild-type enzyme Rattus norvegicus
additional information wild-type non-gastric H,K-ATPase shows a very low affinity for ouabain Rattus norvegicus
ouabagenin inhibition of mutant D312E/S319G/A778P/I795L/F802C, not of the wild-type enzyme Rattus norvegicus
Ouabain inhibition of mutant D312E/S319G/A778P/I795L/F802C, not of the wild-type enzyme Rattus norvegicus
strophanthidin inhibition of mutant D312E/S319G/A778P/I795L/F802C, not of the wild-type enzyme Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
stomach
-
Rattus norvegicus
-

Synonyms

Synonyms Comment Organism
non-gastric H,K-ATPase
-
Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Rattus norvegicus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00017
-
pH 7.0, 37°C, mutant D312E/S319G/A778P/I795L/F802C Rattus norvegicus strophanthidin
0.00031
-
pH 7.0, 37°C, mutant D312E/S319G/A778P/I795L/F802C Rattus norvegicus Ouabain
0.00075
-
pH 7.0, 37°C, mutant D312E/S319G/A778P/I795L/F802C Rattus norvegicus digoxigenin
0.00076
-
pH 7.0, 37°C, mutant D312E/S319G/A778P/I795L/F802C Rattus norvegicus digoxin
0.0028
-
pH 7.0, 37°C, mutant D312E/S319G/A778P/I795L/F802C Rattus norvegicus ouabagenin
0.027
-
pH 7.0, 37°C, mutant D312E/S319G/A778P/I795L/F802C Rattus norvegicus dihydro-ouabain