Activating Compound | Comment | Organism | Structure |
---|---|---|---|
H+ | - |
Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
expression of alpha-subunit of wild-type and mutant enzymes in Spodopera frugiperda Sf9 cell membranes using the baculovirus transfection system | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
D312E/S319G/A778P/I795L/F802C | a ouabain-sensitive mutant of the non-gastric H,K-ATPase, with the mutant enzyme, strophanthidin and dihydroouabain have a higher and digoxin has a lower affinity than ouabain | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
digoxigenin | inhibition of mutant D312E/S319G/A778P/I795L/F802C, not of the wild-type enzyme | Rattus norvegicus | |
digoxin | inhibition of mutant D312E/S319G/A778P/I795L/F802C, not of the wild-type enzyme | Rattus norvegicus | |
dihydro-ouabain | inhibition of mutant D312E/S319G/A778P/I795L/F802C, not of the wild-type enzyme | Rattus norvegicus | |
additional information | wild-type non-gastric H,K-ATPase shows a very low affinity for ouabain | Rattus norvegicus | |
ouabagenin | inhibition of mutant D312E/S319G/A778P/I795L/F802C, not of the wild-type enzyme | Rattus norvegicus | |
Ouabain | inhibition of mutant D312E/S319G/A778P/I795L/F802C, not of the wild-type enzyme | Rattus norvegicus | |
strophanthidin | inhibition of mutant D312E/S319G/A778P/I795L/F802C, not of the wild-type enzyme | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stomach | - |
Rattus norvegicus | - |
Synonyms | Comment | Organism |
---|---|---|
non-gastric H,K-ATPase | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Rattus norvegicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00017 | - |
pH 7.0, 37°C, mutant D312E/S319G/A778P/I795L/F802C | Rattus norvegicus | strophanthidin | |
0.00031 | - |
pH 7.0, 37°C, mutant D312E/S319G/A778P/I795L/F802C | Rattus norvegicus | Ouabain | |
0.00075 | - |
pH 7.0, 37°C, mutant D312E/S319G/A778P/I795L/F802C | Rattus norvegicus | digoxigenin | |
0.00076 | - |
pH 7.0, 37°C, mutant D312E/S319G/A778P/I795L/F802C | Rattus norvegicus | digoxin | |
0.0028 | - |
pH 7.0, 37°C, mutant D312E/S319G/A778P/I795L/F802C | Rattus norvegicus | ouabagenin | |
0.027 | - |
pH 7.0, 37°C, mutant D312E/S319G/A778P/I795L/F802C | Rattus norvegicus | dihydro-ouabain |