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Literature summary for 7.2.2.19 extracted from

  • Nishizawa, T.; Abe, K.; Tani, K.; Fujiyoshi, Y.
    Structural analysis of 2D crystals of gastric H+,K+-ATPase in different states of the transport cycle (2008), J. Struct. Biol., 162, 219-228.
    View publication on PubMed
Search for more literature for 7.2.2.19

Crystallization (Commentary)

Crystallization (Comment) Organism
AlFxADP bound enzyme form, beta-octyl glycoside-solubilized enzyme from SDS-purified membranes by 2D crystallization, dialysis for 12 h to 2 days in presence of Mg2+, glycerol and/or propionate in different compositions, structure determination and analysis by cryoelectron microscopy of the 2D samples, resolution of 14 A Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
Trypsin limited proteolysis, determination of peptide fragment sequences, overview Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Sus scrofa 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + H+/in + K+/out Sus scrofa
-
 ADP + phosphate + H+/out + K+/in
-
 ?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native gastric H+,K+-ATPase partially by enzyme-enriched membrane vesicle preparation using SDS or/and beta-octyl glycoside Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
stomach gastric membrane Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + H+/in + K+/out
-
 Sus scrofa ADP + phosphate + H+/out + K+/in
-
 ?

Synonyms

Synonyms Comment Organism
gastric H+,K+-ATPase
-
 Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Sus scrofa