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Literature summary for 7.2.2.19 extracted from

  • Reenstra, W.W.; Crothers, J.; Forte, J.G.
    The conformation of H,K-ATPase determines the nucleoside triphosphate (NTP) selectivity for active proton transport (2007), Biochemistry, 46, 10145-10152.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ADP activates NTPase activity of the enzyme Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Sus scrofa 16020
-
microsome
-
Sus scrofa
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activates, required for ATPase activity Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + H+/in + K+/out Sus scrofa
-
ADP + phosphate + H+/out + K+/in
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially by preparation of enzyme-enriched microsomal vesicles Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
stomach mucosa Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
NTP hydrolysis activities, overview Sus scrofa
0.626
-
ATP hydrolysis in presence of 10 mM K+ and 0.003 mM valinomycin Sus scrofa
0.641
-
ATP hydrolysis in presence of 10 mM K+, 0.1 mM ADP, and 0.003 mM valinomycin Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + H+/in + K+/out
-
Sus scrofa ADP + phosphate + H+/out + K+/in
-
?
additional information the enzyme also performs K+-dependent NTP hydrolysis, utilizing ATP, GTP, CTP, UTP, and ITP, and an NTP/ADP exchange reaction, overview Sus scrofa ?
-
?

Synonyms

Synonyms Comment Organism
H,K-ATPase
-
Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
assay at Sus scrofa