Literature summary for 7.2.2.19 extracted from

Koenderink, J.B.; Swarts, H.G.P.; Willems, P.H.G.M.; Krieger, E.; De Pont, J.J.H.H.M.
A conformation-specific interhelical salt bridge in the K+ binding site of gastric H,K-ATPase (2004), J. Biol. Chem., 279, 16417-16424.

Search for more literature for 7.2.2.19

Application

Application	Comment	Organism
additional information	the salt bridge between Glu820 and Lys791 is essential for high-affinity K+ binding and the E2 preference of the enzyme	Rattus norvegicus

Protein Variants

Protein Variants	Comment	Organism
E343D	low spontaneous dephosphorylation rate	Rattus norvegicus
E343D/E820Q	low spontaneous dephosphorylation rate	Rattus norvegicus
E820Q	K+-insensitive activity and an E1 preference, E2 form-specific salt bridge between Glu820 and Lys791 is no longer possible	Rattus norvegicus
K791A	K+ affinity is markedly reduced without altering the E2 preference of the enzyme	Rattus norvegicus
K791A/E343D	no K+-independent ATPase activity	Rattus norvegicus
K791A/E343D/E820Q	completely K+-insensitive	Rattus norvegicus
K791A/E820Q	no K+-independent ATPase activity	Rattus norvegicus
K791R	K+-stimulated ATPase acitvity hardly significant	Rattus norvegicus
K791R/E343D	no K+-independent ATPase activity, no K+-stimulated dephosphorylation activity	Rattus norvegicus
K791R/E343D/E820Q	no K+-stimulated dephosphorylation activity	Rattus norvegicus
K791R/E820Q	some K+-independent ATPase activity, no K+-stimulated dephosphorylation activity	Rattus norvegicus
K791S	rather similar properties than the K791A mutant	Rattus norvegicus

Inhibitors

Inhibitors	Comment	Organism	Structure
SCH28080	low concentrations decrease phosphorylation levels of mutants with an E2 preference, whereas it hardly changes with E1-prefering mutants, even increases phosphorylation levels in the E820Q mutant	Rattus norvegicus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K+	high affinity binding site between Glu820 and Lys791	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	P09626	-	-

Synonyms

Synonyms	Comment	Organism
gastric H,K-ATPase	-	Rattus norvegicus

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Release 2023.1 (January 2023)