Cloned (Comment) | Organism |
---|---|
gene mgtE, overexpression of N-terminally His-tagged full-length enzyme and cytosolic domain in Escherichia coli strain C41 (DE3) | Thermus thermophilus |
Crystallization (Comment) | Organism |
---|---|
selenomethionine-labeled full-length enzyme and soluble cytosolic domain in presence and absence of Mg2+, 10 mg/ml full-length protein with 0.1% dodecylmaltoside, by vapour diffusion over 32-34% 2-methyl-2,4-pentanediol, 40 mM magnesium acetate, and 100 mM MES, pH 6.0, cytosolic domain with bound Mg2+ by vapour diffusion over solutions containing 18-22% PEG 400, 0.2M MgCl2, and 0.1M HEPES, pH 7.4, or cytosolic domain in the absence of Mg2+ in 20% PEG 3350, 0.2 M ammonium acetate, and 0.5% octyl-beta-D-glucopyranoside, X-ray diffraction structure determination at 2.3-3.9 A resolution, multiple anomaleous dispersion method | Thermus thermophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | the transmembrane helices are connected by 5 loops, structure, overview | Thermus thermophilus | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Mg2+/out | Thermus thermophilus | Mg21 homeostasis mechanism model, overview | ADP + phosphate + Mg2+/in | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q5SMG8 | gene mgtE | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged cytosolic domain by nickel affinity chromatography, tag cleavage by thrombin, anion exchange chromatography and gel filtration, recombinant N-terminally His-tagged full-length MgtE from Escherichia coli strain C41 (DE3) by nickel affinity chromatography and gel filtration | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Mg2+/out | Mg21 homeostasis mechanism model, overview | Thermus thermophilus | ADP + phosphate + Mg2+/in | - |
? | |
ATP + H2O + Mg2+/out | transport of the fully hydrated Mg2+ ion, one Mg2+ is bound to conserved Asp432 within the solvent-accessible pore of the transmembrane segement, four Mg2+ ions are bound at the interface between the connecting helices and the other domains, Mg2+ binding structure, overview | Thermus thermophilus | ADP + phosphate + Mg2+/in | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Mg2+ transporter | - |
Thermus thermophilus |
MgtE | - |
Thermus thermophilus |