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Literature summary for 7.2.2.14 extracted from
- Bacterial homologs of eukaryotic membrane proteins: the 2-TM-GxN family of Mg2+ transporters (Review) (2007), Mol. Membr. Biol., 24, 351-356.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystal structure analysis from PDB ID BBJ2 at 3.9 A resolution for the whole enzyme, and at 1.8 A resolution for the soluble domain, overview | Thermotoga maritima |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | Alr1p mutants show 25-30% reduced Mg2+ content but fail to accumulate Mg2+ | Saccharomyces cerevisiae |
| additional information | mutation of residues near L294/M291 alters the transport properties of CorA | Thermotoga maritima |
| additional information | selection of Mg2+ transport defect corA mutants by screening for Co2+ resistant strains, since accumulation of Co2+ is tosic for the cells | Escherichia coli |
| additional information | the mgtAmgtCB mutant strain is dependent on CorA for Mg2+ uptake and is completely growth-inhibited by cation hexaamines, Mrs2p mutants can be complemented by bacterial CorA, mrs2 mutants decreases Mg2+ uptake and Mg2+ content in the mitochondria by 62% | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | inhibits Alr1p and Alr2p; inhibits Alr1p and Alr2p | Saccharomyces cerevisiae |  |
| Co(III) hexaamines | inhibit Mrs2p and CorA, inhibition of Ni2+ uptake by CorA | Saccharomyces cerevisiae | |
| Co2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| Mg2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| Mn2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| additional information | no inhibition of Mg2+ or Ni2+ uptake by CorA through Fe2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
| Ni2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| ruthenium (III) hexaamines | inhibit Mrs2p and CorA, inhibition of Ni2+ uptake by CorA | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
Mg2+ | 20°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.015 | - |
Mg2+ | 20°C | Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrial inner membrane | Mrs2p | Saccharomyces cerevisiae | 5743 | - |
| plasma membrane | Alr1p | Saccharomyces cerevisiae | 5886 | - |
| plasma membrane | Alr2p | Saccharomyces cerevisiae | 5886 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + Mg2+/out | Salmonella enterica subsp. enterica serovar Typhimurium | - |
ADP + phosphate + Mg2+/in | - |
? | |
| ATP + H2O + Mg2+/out | Thermotoga maritima | - |
ADP + phosphate + Mg2+/in | - |
? | |
| ATP + H2O + Mg2+/out | Saccharomyces cerevisiae | - |
ADP + phosphate + Mg2+/in | - |
? | |
| ATP + H2O + Mg2+/out | Saccharomyces cerevisiae | at high Mg2+ levels Alr1p is rapidly degraded by the ubiquitin dependent pathway | ADP + phosphate + Mg2+/in | - |
? | |
| ATP + H2O + Mg2+/out | Saccharomyces cerevisiae | Mg2+ uptake by Mrs2p is functionally regulated in the mitochondria | ADP + phosphate + Mg2+/in | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
gene corA | - |
| Saccharomyces cerevisiae | P43553 | Alr2p; gene alr2 | - |
| Saccharomyces cerevisiae | Q02783 | Mrs2p; gene mrs2 | - |
| Saccharomyces cerevisiae | Q08269 | Alr1p; gene alr1 | - |
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
gene corA | - |
| Thermotoga maritima | Q9WZ31 | gene corA | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + H2O + Mg2+[side 1] = ADP + phosphate + Mg2+[side 2] | Mg2+ transport mechanism, structure-function relationship, overview | Thermotoga maritima |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | nonrepressible constitutive expression of CorA | Salmonella enterica subsp. enterica serovar Typhimurium | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + Co2+/out | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ADP + phosphate + Co2+/in | - |
? | |
| ATP + H2O + Co2+/out | - |
Escherichia coli | ADP + phosphate + Co2+/in | - |
? | |
| ATP + H2O + Mg2+/out | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ADP + phosphate + Mg2+/in | - |
? | |
| ATP + H2O + Mg2+/out | - |
Escherichia coli | ADP + phosphate + Mg2+/in | - |
? | |
| ATP + H2O + Mg2+/out | - |
Thermotoga maritima | ADP + phosphate + Mg2+/in | - |
? | |
| ATP + H2O + Mg2+/out | - |
Saccharomyces cerevisiae | ADP + phosphate + Mg2+/in | - |
? | |
| ATP + H2O + Mg2+/out | at high Mg2+ levels Alr1p is rapidly degraded by the ubiquitin dependent pathway | Saccharomyces cerevisiae | ADP + phosphate + Mg2+/in | - |
? | |
| ATP + H2O + Mg2+/out | Mg2+ uptake by Mrs2p is functionally regulated in the mitochondria | Saccharomyces cerevisiae | ADP + phosphate + Mg2+/in | - |
? | |
| ATP + H2O + Ni2+/out | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ADP + phosphate + Ni2+/in | - |
? | |
| additional information | no transport of Fe2+, Mn2+, or Ca2+ | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | secondary structure determination and analysis | Thermotoga maritima |
| oligomer | Alr1p is at least a tetramer, Alr1p and Alr2p form homooligomers, but heteroligomers when overexpressed, the oligomerization of Alr1p results in a dominant negative action on the protein | Saccharomyces cerevisiae |
| oligomer | Alr2p forms homooligomers, but heteroligomers when overexpressed | Saccharomyces cerevisiae |
| pentamer | CorA | Escherichia coli |
| pentamer | CorA | Thermotoga maritima |
| pentamer | CorA | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ALR1 | - |
Saccharomyces cerevisiae |
| ALR2 | - |
Saccharomyces cerevisiae |
| CorA | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| CorA | - |
Escherichia coli |
| CorA | - |
Thermotoga maritima |
| More | the enzyme belongs to the 2-TM-GxN family of Mg2+ transporters | Escherichia coli |
| More | the enzyme belongs to the 2-TM-GxN family of Mg2+ transporters | Thermotoga maritima |
| More | the enzyme belongs to the 2-TM-GxN family of Mg2+ transporters | Saccharomyces cerevisiae |
| Mrs2p | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
| 20 | - |
assay at | Escherichia coli |
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