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Literature summary for 7.2.2.13 extracted from
- Overview: the Na,K-pump (1988), Methods Enzymol., 156, 1-25.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| oligomycin | not completely inhibits but decreases the rate of turnover of transport and of the hydrolysis reaction. Oligomycin affects the extracellular side of the system, Na+ on the cytoplasmic side of the system is necessary for the effect, oligomycin decreases the rate of transition from the Na+ form to the K+ form of the enzyme, but has no effect on the rate of the reverse reaction | Mammalia |  |
| oubain | K+ protects against inhibition, probably due to phosphorylating effect | Mammalia | |
| vanadate | - |
Mammalia | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Mammalia | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cs+ | the Na,K pump is activated by the combined effect of Na+ on the cytoplasmic side and of K+ on the extracellular side of the membrane. K+ can be replaced by the following monovalent cations in the order of decreasing activation: K+, Rb+, NH4+, Cs+, Li, Na+ | Mammalia | |
| K+ | the Na,K pump is activated by the combined effect of Na+ on the cytoplasmic side and of K+ on the extracellular side of the membrane. K+ can be replaced by the following monovalent cations in the order of decreasing activation: Rb+, NH4+, Cs+, Li, Na+ | Mammalia | |
| Li+ | the Na,K pump is activated by the combined effect of Na+ on the cytoplasmic side and of K+ on the extracellular side of the membrane. K+ can be replaced by the following monovalent cations in the order of decreasing activation: K+, Rb+, NH4+, Cs+, Li, Na+ | Mammalia | |
| Na+ | the Na,K pump is activated by the combined effect of Na+ on the cytoplasmic side and of K+ on the extracellular side of the membrane. Na+ cannot be replaced by any other monovalent cation. K+ can be replaced by the following monovalent cations in the order of decreasing activation: K+, Rb+, NH4+, Cs+, Li, Na+ | Mammalia | |
| NH4+ | the Na,K pump is activated by the combined effect of Na+ on the cytoplasmic side and of K+ on the extracellular side of the membrane. K+ can be replaced by the following monovalent cations in the order of decreasing activation: K+, Rb+, NH4+, Cs+, Li, Na+ | Mammalia | |
| Rb+ | the Na,K pump is activated by the combined effect of Na+ on the cytoplasmic side and of K+ on the extracellular side of the membrane. K+ can be replaced by the following monovalent cations in the order of decreasing activation: K+, Rb+, NH4+, Cs+, Li, Na+ | Mammalia | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
x * 100000, alpha-subunit, + x * 38000, beta-subunit, the ratio of alpha: beta is 1:1 | Mammalia |
| 100000 | - |
x * 100000, alpha-subunit, + x * 38000, beta-subunit, the ratio of alpha: beta is 1:1 | Mammalia |
| 379000 | - |
equilibrium sedimentation | shark |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + Na+/in + K+/out | Mammalia | creation of a electrochemical gradient of Na+ ions into and K+ ions out of the cell | ADP + phosphate + Na+/out + K+/in | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mammalia | - |
- |
- |
| shark | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the beta subunit is a glycoprotein | Mammalia |
| side-chain modification | required for activity, removal of lipids leads to inactivation and readdition of the lipids results in reactivation of the enzyme | Mammalia |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
shark |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + Na+/in + K+/out | - |
shark | ADP + phosphate + Na+/out + K+/in | - |
? | |
| ATP + H2O + Na+/in + K+/out | for each ATP molecule hydrolyzed three Na+ ions are transported out of and two K+ ions into the cell | Mammalia | ADP + phosphate + Na+/out + K+/in | - |
? | |
| ATP + H2O + Na+/in + K+/out | creation of a electrochemical gradient of Na+ ions into and K+ ions out of the cell | Mammalia | ADP + phosphate + Na+/out + K+/in | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 100000, alpha-subunit, + x * 38000, beta-subunit, the ratio of alpha: beta is 1:1 | Mammalia |
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