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Literature summary for 7.2.2.12 extracted from
- A novel regulatory metal binding domain is present in the C terminus of Arabidopsis Zn2+-ATPase HMA2 (2006), J. Biol. Chem., 281, 33881-33891.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Arabidopsis thaliana HMA2 and truncated forms of the protein are prepared using the bacterial expression vector pPRIBA1 and the yeast expression vector pYES2/CT | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C-MBD | HMA2 C-terminal metal binding domain | Arabidopsis thaliana |
| DELTAC-HMA2 | mutant, lacking the 244 C-terminal amino acids | Arabidopsis thaliana |
| DELTANC-HMA2 | mutant, lacking the N-terminal first 75 amino acids and the 244 C-terminal amino acids | Arabidopsis thaliana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Arabidopsis thaliana | 16020 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + Zn2+/in | Arabidopsis thaliana | - |
ADP + phosphate + Zn2+/out | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| membranes from yeast are prepared, purification of the HMA2 C-terminal metal binding domain is performed using a Strep-Tactin Superflow column | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + Zn2+/in | - |
Arabidopsis thaliana | ADP + phosphate + Zn2+/out | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HMA2 | - |
Arabidopsis thaliana |
| Zn2+-ATPase | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
ATPase activity assay | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
ATPase activity assay | Arabidopsis thaliana |
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Release 2023.1 (January 2023)
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