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Literature summary for 7.2.2.12 extracted from

  • Dutta, S.J.; Liu, J.; Stemmler, A.J.; Mitra, B.
    Conservative and nonconservative mutations of the transmembrane CPC motif in ZntA: effect on metal selectivity and activity (2007), Biochemistry, 46, 3692-3703.
    View publication on PubMed
Search for more literature for 7.2.2.12

Cloned(Commentary)

Cloned (Comment) Organism
into the pBAD/Myc-Hic C or pBAD/Myc-His A vector for expression in the Escherichia coli strain LMG194(zntA::cat) Escherichia coli

Protein Variants

Protein Variants Comment Organism
C392A mutant for evaluating the importance of the cysteine residue of the conserved 392CPC394 motif in metal ion binding Escherichia coli
C392H mutant for evaluating the importance of the cysteine residue of the conserved 392CPC394 motif in metal ion binding Escherichia coli
C392H/C394H mutant for evaluating the importance of the cysteine residue of the conserved 392CPC394 motif in metal ion binding Escherichia coli
C392S mutant for evaluating the importance of the cysteine residue of the conserved 392CPC394 motif in metal ion binding Escherichia coli
C392S/C394S mutant for evaluating the importance of the cysteine residue of the conserved 392CPC394 motif in metal ion binding Escherichia coli
C394A mutant for evaluating the importance of the cysteine residue of the conserved 392CPC394 motif in metal ion binding Escherichia coli
C394S mutant for evaluating the importance of the cysteine residue of the conserved 392CPC394 motif in metal ion binding Escherichia coli
P393A mutant, shows no activity and can not bind any metal ion at the transmembrane site Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0021
-
 Ni2+ wild-type ZntA Escherichia coli
0.0031
-
 Cd2+ wild-type ZntA Escherichia coli
0.0034
-
 Cu2+ wild-type ZntA Escherichia coli
0.004
-
 Co2+ wild-type ZntA Escherichia coli
0.0051
-
 Zn2+ wild-type ZntA Escherichia coli
0.0061
-
 Pb2+ wild-type ZntA Escherichia coli
0.007
-
 Co2+ mutant C392H Escherichia coli
0.007
-
 Zn2+ mutant C392S/C394S Escherichia coli
0.007
-
 Cu2+ mutant C394S Escherichia coli
0.007
-
 Co2+ mutant C394S Escherichia coli
0.007
-
 Ni2+ mutant C394S Escherichia coli
0.008
-
 Co2+ mutant C394H Escherichia coli
0.009
-
 Zn2+ mutant C392S Escherichia coli
0.009
-
 Ni2+ mutant C394H Escherichia coli
0.009
-
 Cd2+ mutant C394S Escherichia coli
0.01
-
 Zn2+ mutant C392H Escherichia coli
0.01
-
 Cu2+ mutant C392H/C394H Escherichia coli
0.01
-
 Cd2+ mutant C392H/C394H Escherichia coli
0.01
-
 Cu2+ mutant C392S Escherichia coli
0.01
-
 Cd2+ mutant C394H Escherichia coli
0.011
-
 Cd2+ mutant C392S Escherichia coli
0.012
-
 Cd2+ mutant C392H Escherichia coli
0.012
-
 Co2+ mutant C392S Escherichia coli
0.012
-
 Zn2+ mutant C394H Escherichia coli
0.013
-
 Cu2+ mutant C392H Escherichia coli
0.013
-
 Ni2+ mutant C392H Escherichia coli
0.013
-
 Zn2+ mutant C392H/C394H Escherichia coli
0.013
-
 Ni2+ mutant C392H/C394H Escherichia coli
0.013
-
 Ni2+ mutant C392S Escherichia coli
0.013
-
 Cd2+ mutant C392S/C394S Escherichia coli
0.013
-
 Cu2+ mutant C394H Escherichia coli
0.014
-
 Co2+ mutant C392H/C394H Escherichia coli
0.014
-
 Zn2+ mutant C394S Escherichia coli
0.018
-
 Co2+ mutant C392S/C394S Escherichia coli
0.019
-
 Cu2+ mutant C392S/C394S Escherichia coli
0.066
-
 Co2+ + thiolate, mutant C392H/C394H Escherichia coli
0.075
-
 Co2+ + thiolate, wild-type ZntA Escherichia coli
0.08
-
 Ni2+ + thiolate, mutant C392H Escherichia coli
0.08
-
 Ni2+ + thiolate, mutant C392S Escherichia coli
0.083
-
 Zn2+ + thiolate, mutant C392H/C394H Escherichia coli
0.083
-
 Ni2+ + thiolate, mutant C394H Escherichia coli
0.085
-
 Cu2+ + thiolate, wild-type ZntA Escherichia coli
0.091
-
 Cd2+ + thiolate, mutant C392H/C394H Escherichia coli
0.092
-
 Co2+ + thiolate, mutant C394H Escherichia coli
0.096
-
 Co2+ + thiolate, mutant C392S/C394S Escherichia coli
0.099
-
 Zn2+ + thiolate, mutant C392S/C394S Escherichia coli
0.104
-
 Co2+ + thiolate, mutant C394S Escherichia coli
0.105
-
 Cu2+ + thiolate, mutant C392H/C394H Escherichia coli
0.105
-
 Zn2+ + thiolate, wild-type ZntA Escherichia coli
0.11
-
 Cd2+ + thiolate, mutant C394H Escherichia coli
0.114
-
 Cd2+ + thiolate, mutant C392H Escherichia coli
0.116
-
 Ni2+ + thiolate, mutant C392H/C394H Escherichia coli
0.117
-
 Ni2+ + thiolate, mutant C394S Escherichia coli
0.123
-
 Cd2+ + thiolate, wild-type ZntA Escherichia coli
0.124
-
 Cu2+ + thiolate, mutant C392S Escherichia coli
0.126
-
 Co2+ + thiolate, mutant C392S Escherichia coli
0.127
-
 Cu2+ + thiolate, mutant C392S/C394S Escherichia coli
0.131
-
 Zn2+ + thiolate, mutant C392H Escherichia coli
0.131
-
 Zn2+ + thiolate, mutant C394H Escherichia coli
0.133
-
 Co2+ + thiolate, mutant C392H Escherichia coli
0.139
-
 Cd2+ + thiolate, mutant C392S/C394S Escherichia coli
0.141
-
 Zn2+ + thiolate, mutant C392S Escherichia coli
0.145
-
 Cu2+ + thiolate, mutant C394S Escherichia coli
0.148
-
 Cd2+ + thiolate, mutant C392S Escherichia coli
0.15
-
 Pb2+ + thiolate, wild-type ZntA Escherichia coli
0.152
-
 Cd2+ + thiolate, mutant C394S Escherichia coli
0.158
-
 Cu2+ + thiolate, mutant C392H Escherichia coli
0.169
-
 Ni2+ + thiolate, wild-type ZntA Escherichia coli
0.171
-
 Cu2+ + thiolate, mutant C394H Escherichia coli
0.182
-
 Zn2+ + thiolate, mutant C394S Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Escherichia coli 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + Cd2+/in Escherichia coli
-
 ADP + phosphate + Cd2+/out
-
 ?
ATP + H2O + Co2+/in Escherichia coli
-
 ADP + phosphate + Co2+/out
-
 ?
ATP + H2O + Cu2+/in Escherichia coli
-
 ADP + phosphate + Cu2+/out
-
 ?
ATP + H2O + Ni2+/in Escherichia coli
-
 ADP + phosphate + Ni2+/out
-
 ?
ATP + H2O + Pb2+/in Escherichia coli
-
 ADP + phosphate + Pb2+/out
-
 ?
ATP + H2O + Zn2+/in Escherichia coli
-
 ADP + phosphate + Zn2+/out
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using a Talon-affinity column Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Cd2+/in
-
 Escherichia coli ADP + phosphate + Cd2+/out
-
 ?
ATP + H2O + Co2+/in
-
 Escherichia coli ADP + phosphate + Co2+/out
-
 ?
ATP + H2O + Cu2+/in
-
 Escherichia coli ADP + phosphate + Cu2+/out
-
 ?
ATP + H2O + Ni2+/in
-
 Escherichia coli ADP + phosphate + Ni2+/out
-
 ?
ATP + H2O + Pb2+/in
-
 Escherichia coli ADP + phosphate + Pb2+/out
-
 ?
ATP + H2O + Zn2+/in
-
 Escherichia coli ADP + phosphate + Zn2+/out
-
 ?

Synonyms

Synonyms Comment Organism
ZntA
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 ATPase activity assay Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 ATPase activity assay Escherichia coli