Cloned (Comment) | Organism |
---|---|
mutant enzymes C59A/C62A and C392A/C394A | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C392A/C394A | mutant enzyme binds metal ions only at the N-terminal site | Escherichia coli |
C59A/C62A | mutant enzyme in which the N-terminal metal-binding site is disabled by site-specific mutagenesis, can bind only one metal ion | Escherichia coli |
additional information | DELTA-ZntA, in which the N-terminal domain is deleted can bind only one metal ion | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cs2+ | ZntA confers resistance specifically to Pb2+, Zn2+, and Cd2+ in Escherichia coli. Wild-type ZntA binds two metal ions with high affinity, one in the N-terminal domain and another in the transmembrane domain | Escherichia coli | |
Pb2+ | ZntA confers resistance specifically to Pb2+, Zn2+, and Cd2+ in Escherichia coli. Wild-type ZntA binds two metal ions with high affinity, one in the N-terminal domain and another in the transmembrane domain | Escherichia coli | |
Zn2+ | ZntA confers resistance specifically to Pb2+, Zn2+, and Cd2+ in Escherichia coli. Wild-type ZntA binds two metal ions with high affinity, one in the N-terminal domain and another in the transmembrane domain. Wild-type ZntA binds two metal ions with high affinity, one in the N-terminal domain and another in the transmembrane domain | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Cd2+/in | Escherichia coli | ZntA confers resistance specifically to Pb2+, Zn2+, and Cd2+ in Escherichia coli | ADP + phosphate + Cd2+/out | - |
? | |
ATP + H2O + Pb2+/in | Escherichia coli | ZntA confers resistance specifically to Pb2+, Zn2+, and Cd2+ in Escherichia coli | ADP + phosphate + Pb2+/out | - |
? | |
ATP + H2O + Zn2+/in | Escherichia coli | ZntA confers resistance specifically to Pb2+, Zn2+, and Cd2+ in Escherichia coli | ADP + phosphate + Zn2+/out | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
mutant enzymes C59A/C62A and C392A/C394A | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Cd2+/in | ZntA confers resistance specifically to Pb2+, Zn2+, and Cd2+ in Escherichia coli | Escherichia coli | ADP + phosphate + Cd2+/out | - |
? | |
ATP + H2O + Pb2+/in | ZntA confers resistance specifically to Pb2+, Zn2+, and Cd2+ in Escherichia coli | Escherichia coli | ADP + phosphate + Pb2+/out | - |
? | |
ATP + H2O + Zn2+/in | ZntA confers resistance specifically to Pb2+, Zn2+, and Cd2+ in Escherichia coli | Escherichia coli | ADP + phosphate + Zn2+/out | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ZntA | - |
Escherichia coli |