Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-cysteine | activates the enzyme and mutant lacking 100 residues | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | Construction of a mutant lacking the N-terminal domain and both the CCCDXXC and GXXCXXC. The activity of the mutant is similar to the wild-type. The Km for ATP is unchanged. The function of the amino-terminal domain may be to increase the overall catalytic rate by increasing the rate of binding of specific metal ions to the transporter. | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
Cd2+ | pH 7.0, 37°C, mutant lacking the N-terminal domain and both the CCCDXXC and GXXCXXC | Escherichia coli | |
0.005 | - |
Pb2+ | pH 7.0, 37°C, enzyme | Escherichia coli | |
0.0055 | - |
Cd2+ | pH 7.0, 37°C, enzyme | Escherichia coli | |
0.009 | - |
Zn2+ | pH 7.0, 37°C, mutant lacking the N-terminal domain and both the CCCDXXC and GXXCXXC | Escherichia coli | |
0.01 | - |
Zn2+ | pH 7.0, 37°C, enzyme | Escherichia coli | |
0.012 | - |
Pb2+ | pH 7.0, 37°C, mutant lacking the N-terminal domain and both the CCCDXXC and GXXCXXC | Escherichia coli | |
0.064 | - |
ATP | pH 7.0, 37°C, mutant lacking the N-terminal domain and both the CCCDXXC and GXXCXXC | Escherichia coli | |
0.075 | - |
ATP | pH 7.0, 37°C, enzyme | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69000 | - |
the mutant lacking the N-terminal domain and both the CCCDXXC and GXXCXXC, SDS-PAGE | Escherichia coli |
80000 | - |
wild-type, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
the recombinant mutant lacking the N-terminal domain and both the CCCDXXC and GXXCXXC | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Cd2+/in | - |
Escherichia coli | ADP + phosphate + Cd2+/out | - |
? | |
ATP + H2O + Pb2+/in | - |
Escherichia coli | ADP + phosphate + Pb2+/out | - |
? | |
ATP + H2O + Zn2+/in | - |
Escherichia coli | ADP + phosphate + Zn2+/out | - |
? |