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Literature summary for 7.2.2.12 extracted from
- Expression and mutagenesis of ZntA, a zinc-transporting P-type ATPase from Escherichia coli (1999), Biochemistry, 38, 14109-14116.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E470A | metal ion-stimulated activity is reduced, about 30-40%. Mutant is phosphorylated more strongly in comparison to the wild type enzyme | Escherichia coli |
| H475Q | metal ion-stimulated activity is reduced, about 30-40%. Mutant is phosphorylated more weakly in comparison to the wild type enzyme. Mutation affects the reaction with ATP and phosphate and stabilizes the enzyme in a dephosphorylated state | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | prevents Zn2+ activation when present in 2fold excess over Zn2+ | Escherichia coli |  |
| vanadate | - |
Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Escherichia coli | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | activates ATPase activity | Escherichia coli | |
| Cu2+ | enhances phosphorylation, it cannot activate the ATPase | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 92000 | - |
1 * 92000, also detection of a dimeric form of 190000 Da, SDS-PAGE | Escherichia coli |
| 92000 | - |
2 * 92000, also detection of a monomeric form of 92000 Da, SDS-PAGE | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + Ag+/in | - |
Escherichia coli | ADP + phosphate + Ag+/out | - |
? | |
| ATP + H2O + Cd2+/in | - |
Escherichia coli | ADP + phosphate + Cd2+/out | - |
? | |
| ATP + H2O + Pb2+/in | - |
Escherichia coli | ADP + phosphate + Pb2+/out | - |
? | |
| ATP + H2O + Zn2+/in | - |
Escherichia coli | ADP + phosphate + Zn2+/out | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 92000, also detection of a monomeric form of 92000 Da, SDS-PAGE | Escherichia coli |
| monomer | 1 * 92000, also detection of a dimeric form of 190000 Da, SDS-PAGE | Escherichia coli |
| More | - |
Escherichia coli |
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