Literature summary for 7.2.2.10 extracted from

Sacchetto, R.; Bertipaglia, I.; Giannetti, S.; Cendron, L.; Mascarello, F.; Damiani, E.; Carafoli, E.; Zanotti, G.
Crystal structure of sarcoplasmic reticulum Ca2+-ATPase (SERCA) from bovine muscle (2012), J. Struct. Biol., 178, 38-44.

Search for more literature for 7.2.2.10

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 220 mM sodium acetate, 4% (v/v) tert-butanol, 5 mM 2-mercaptoethanol, 15% (v/v) glycerol, and 6-7% (w/v) PEG 6000	Oryctolagus cuniculus
hanging drop vapor diffusion method, using 220 mM sodium acetate, 4% (v/v) tert-butanol, 5 mM 2-mercaptoethanol, 15% (v/v) glycerol, and 6-7% (w/v) PEG 6000	Bos taurus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
sarcoplasmic reticulum	-	Oryctolagus cuniculus	16529	-
sarcoplasmic reticulum	-	Bos taurus	16529	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
110000	-	x * 110000, isoform SERCA1, SDS-PAGE	Oryctolagus cuniculus
110000	-	x * 110000, isoform SERCA1, SDS-PAGE	Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + 2 Ca2+[cytoplasm side]	Oryctolagus cuniculus	-	ADP + phosphate + 2 Ca2+[lumen side]	-	?
ATP + H2O + 2 Ca2+[cytoplasm side]	Bos taurus	-	ADP + phosphate + 2 Ca2+[lumen side]	-	?

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	Q0VCY0	-	-
Oryctolagus cuniculus	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
nitrosylation	at Tyr122	Oryctolagus cuniculus
nitrosylation	at Tyr122	Bos taurus

Source Tissue

Source Tissue	Comment	Organism	Textmining
muscle	-	Oryctolagus cuniculus	-
muscle	-	Bos taurus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
6.5	-	at pH 7.2 and 37°C	Bos taurus
10.06	-	at pH 7.2 and 37°C	Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + 2 Ca2+[cytoplasm side]	-	Oryctolagus cuniculus	ADP + phosphate + 2 Ca2+[lumen side]	-	?
ATP + H2O + 2 Ca2+[cytoplasm side]	-	Bos taurus	ADP + phosphate + 2 Ca2+[lumen side]	-	?

Subunits

Subunits	Comment	Organism
?	x * 110000, isoform SERCA1, SDS-PAGE	Oryctolagus cuniculus
?	x * 110000, isoform SERCA1, SDS-PAGE	Bos taurus

Synonyms

Synonyms	Comment	Organism
sarcoplasmic/endoplasmic reticulum Ca2+-ATPase	-	Oryctolagus cuniculus
sarcoplasmic/endoplasmic reticulum Ca2+-ATPase	-	Bos taurus
SERCA pump	-	Oryctolagus cuniculus
SERCA pump	-	Bos taurus
SERCA1	isoform	Oryctolagus cuniculus
SERCA1	isoform	Bos taurus

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Release 2023.1 (January 2023)