Literature summary for 7.2.2.10 extracted from

Johnson, N.A.; Liu, F.; Weeks, P.D.; Hentzen, A.E.; Kruse, H.P.; Parker, J.J.; Laursen, M.; Nissen, P.; Costa, C.J.; Gatto, C.
A tomato ER-type Ca2+-ATPase, LCA1, has a low thapsigargin-sensitivity and can transport manganese (2009), Arch. Biochem. Biophys., 481, 157-168.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in a Saccharomyces cerevisiae triple mutant defective in both a Golgi and vacuolar Ca2+ pump, strain K616 and strain HI227	Solanum lycopersicum

Protein Variants

Protein Variants	Comment	Organism
D366A	catalytically inactive	Solanum lycopersicum

Inhibitors

Inhibitors	Comment	Organism	Structure
cyclopiazonic acid	highly sensitive to the specific inhibitor cyclopiazonic acid	Solanum lycopersicum
eosin	potent inhibitor	Solanum lycopersicum
thapsigargin	only mildly sensitive to the ER-type pump inhibitor thapsigargin	Solanum lycopersicum
vanadate	potent inhibitor	Solanum lycopersicum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	dependent on	Solanum lycopersicum
Mn2+	LCA1 may also act as a Mn2+ pump, consistent with a possible role in secretory pathway divalent cation homeostasis	Solanum lycopersicum

Organism

Organism	UniProt	Comment	Textmining
Solanum lycopersicum	Q42883	-	-

Storage Stability

Storage Stability	Organism
-80°C, the activity of LCA1 prepared from yeast cells stored at -80°C is dramatically reduced when compared to LCA1 prepared from freshly harvested cells	Solanum lycopersicum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + Ca2+/cis	-	Solanum lycopersicum	ADP + phosphate + Ca2+/trans	-	?

Synonyms

Synonyms	Comment	Organism
LCA1	ER-type Ca2+-ATPase	Solanum lycopersicum

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.00016	-	in 1 mM EGTA, 130 mM NaCl, 3 mM MgCl2, at 37°C	Solanum lycopersicum	cyclopiazonic acid
0.000635	-	recombinant enzyme expressed in Saccharomyces cerevisiae strain HI227, in 1 mM EGTA, 130 mM NaCl, 3 mM MgCl2, at 37°C	Solanum lycopersicum	thapsigargin
0.00095	-	recombinant enzyme expressed in Saccharomyces cerevisiae strain K616, in 1 mM EGTA, 130 mM NaCl, 3 mM MgCl2, at 37°C	Solanum lycopersicum	thapsigargin
0.0036	-	in 1 mM EGTA, 130 mM NaCl, 3 mM MgCl2, at 37°C	Solanum lycopersicum	eosin
0.0057	-	in 1 mM EGTA, 130 mM NaCl, 3 mM MgCl2, at 37°C	Solanum lycopersicum	vanadate

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Release 2023.1 (January 2023)