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Literature summary for 7.2.2.10 extracted from
- Improved expression and characterization of Ca2+-ATPase and phospholamban in High-Five cells (2004), Protein Expr. Purif., 34, 56-67.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type SERCA2a in insect cell membranes using the baculovirus transfection system, process optimization, co-expression of phospholamban, overview | Canis lupus familiaris |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| A23187 | Ca2+ ionophore | Canis lupus familiaris |  |
| phospholamban | complex formation with the recombinant SERCA2a in transfected insect cells, regulatory function, overiew | Canis lupus familiaris |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics of Ca2+ uptake and efflux, overview | Canis lupus familiaris |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Canis lupus familiaris | 16020 | - |
| sarcoplasmic reticulum | - |
Canis lupus familiaris | 16529 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | dependent on | Canis lupus familiaris | |
| Mg2+ | required | Canis lupus familiaris | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + Ca2+/cis | Canis lupus familiaris | - |
ADP + phosphate + Ca2+/trans | - |
? | |
| additional information | Canis lupus familiaris | the enzyme performs ATP-dependent Ca2+ transport and Ca2+-dependent ATPase activity, functional regulation by phospholamban | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Canis lupus familiaris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant SERCA2a 3.61fold from insect cell microsomes | Canis lupus familiaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cardiac muscle | - |
Canis lupus familiaris | - |
| heart | - |
Canis lupus familiaris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
Ca2+ uptake and efflux activity of recombinant enzyme in membrane vesicles of transfected insect cells | Canis lupus familiaris |
| 0.49 | - |
purified recombinant SERCA2a | Canis lupus familiaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + Ca2+/cis | - |
Canis lupus familiaris | ADP + phosphate + Ca2+/trans | - |
? | |
| ATP + H2O + Ca2+/cis | recombinant enzyme | Canis lupus familiaris | ADP + phosphate + Ca2+/trans | - |
? | |
| additional information | the enzyme performs ATP-dependent Ca2+ transport and Ca2+-dependent ATPase activity, functional regulation by phospholamban | Canis lupus familiaris | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Ca2+-ATPase | - |
Canis lupus familiaris |
| SERCA2a | - |
Canis lupus familiaris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Canis lupus familiaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Canis lupus familiaris |
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Release 2023.1 (January 2023)
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