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Literature summary for 7.2.2.10 extracted from
- Functional comparison between secretory pathway Ca2+/Mn2+-ATPase (SPCA) 1 and sarcoplasmic reticulum Ca2+-ATPase (SERCA) 1 isoforms by steady-state and transient kinetic analyses (2005), J. Biol. Chem., 280, 39124-39134.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| genes ATP2C genes encoding isozymes 1a-d, expression of isozymes 1a-1d using an SV40 vector, in HEK-293 and COS-1 cells | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| vanadate | inhibits the phosphorylation reaction; inhibits the phosphorylation reaction | Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | ATPase and phosphorylation kinetics, kinetics of activation by Ca2+, and binding and transport of Ca2+ | Homo sapiens | |
| additional information | - |
additional information | ATPase and phosphorylation kinetics, kinetics of isozymes of activation by Ca2+, and binding and transport of Ca2+ | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| sarcoplasmic reticulum | - |
Homo sapiens | 16529 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Homo sapiens | |
| Ca2+ | quantitative Ca2+ flux analysis of recombinant isozymes in COS-1 cells permeabilized by saponin | Homo sapiens | |
| Mg2+ | - |
Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + Ca2+/cis | Homo sapiens | ATPase activity and Ca2+ transport activity | ADP + phosphate + Ca2+/trans | - |
? | |
| additional information | Homo sapiens | the enzyme is important in the secretory pathway | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
isozyme 1 | - |
| Homo sapiens | P98194 | isozymes SPCA1a-SPCA1d; isozymes 1a-d | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| skeletal muscle | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
Ca2+ ionophore A23187 effect, Ca2+ transport rate isozymes, overview | Homo sapiens |
| additional information | - |
Ca2+ ionophore A23187 effect, Ca2+ transport rate, overview | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + Ca2+/cis | ATPase activity and Ca2+ transport activity | Homo sapiens | ADP + phosphate + Ca2+/trans | - |
? | |
| additional information | the enzyme is important in the secretory pathway | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ATP-dependent Ca(2+) pump PMR1 | - |
Homo sapiens |
| ATPase 2C1 | - |
Homo sapiens |
| Ca2+/Mn2+-ATPase 1 | - |
Homo sapiens |
| calcium-transporting ATPase type 2C member 1 | - |
Homo sapiens |
| sarcoplasmic reticulum Ca2+-ATPase 1 | - |
Homo sapiens |
| SERCA 1 | - |
Homo sapiens |
| SPCA1 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Ca2+ binding transition rate | Homo sapiens | |
| additional information | - |
additional information | Ca2+ binding transition rates of isozymes, overview | Homo sapiens | |
| 3.5 | - |
ATP | ATPase turnover rate, pH 7.0, 37°C, isozyme SPCA1a | Homo sapiens | |
| 15.9 | - |
ATP | ATPase turnover rate, pH 7.0, 37°C, isozyme SPCA1d | Homo sapiens | |
| 20.27 | - |
ATP | ATPase turnover rate, pH 7.0, 37°C, isozyme SPCA1a | Homo sapiens | |
| 22.5 | - |
ATP | ATPase turnover rate, pH 7.0, 37°C, isozyme SPCA1b | Homo sapiens | |
| 27.44 | - |
ATP | ATPase turnover rate, pH 7.0, 37°C, isozyme SPCA1d | Homo sapiens | |
| 130 | - |
ATP | ATPase turnover rate, pH 7.0, 37°C, isozyme SERCA1a | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Homo sapiens |
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